Login | Register
Kazakh National Agrarian Research University ISSN 2304-3334 (Print)  ·  ISSN 2304-3334 (Online)
submit an article
Menu
submit an article
AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

COMPARATIVE CHARACTERISTICS OF MICROTUBERS OF POTATO VARIETY GALA TRANSGENIC LINES

Anna Nizkorodova 1 , Ruslan Kryldakov 1 , Nurlybek Karimov 1 , Nailya Polimbetova 1 , Bulat Iskakov 1

1 RSE “Institute of Molecular Biology and Biochemistry named after M.A. Aitkhozhin”

doi.org/10.37884/3-2025/14 pp. 155-165 Admitted 11.07.2025 Published 30.09.2025

Abstract

Microtubers play an important role in seed potato production technology, as they have great advantages in storage, transportation and mechanization due to their small size and weight. Production of microtubers under sterile conditions is a good way to produce healthy seed material. In this work, we carried out a comparative analysis of potato microtubers of the Gala variety (NORIKA GMBH, Germany) and a number of transgenic lines obtained on the basis of this variety. Transgenic plants were previously obtained as part of a study on the use of the HIGS (host-induced gene silencing) approach to control the potato and tomato plant pathogen Phytophthora infestans. The implementation of the HIGS method leads to expression of short interfering RNAs (siRNA) in the transgenic plants. Such plants when infected with a pathogen cause silencing of the pathogen’s genes of its effector, which prevents germination and reproduction of the pathogenic organism in the plant. The basis of this method is modification of plants with relatively short transgenic cassettes that do not encode target proteins. Therefore, it is expected that such transgenic plants should not have phenotypic and physiological differences from plants of the original variety. We obtained microtubers of non-modified plants and six lines of transgenic plants carrying three types of transgenic inserts. We compared such properties of the obtained microtubers as weight, dormancy period, appearance, rate and percentage of germination in soil. The obtained data showed the absence of statistically significant differences between transgenic and non-transgenic microtubers for all studied properties, except for the rate of germination in soil. Two of six studied transgenic lines showed an increase in the germination rate in open ground by ~ 1.6 times.

RNA interference Phytophthora infestans Host Induced Gene Silencing siRNA transgenic plants Avr3a Avh246 PITG_03155

01 Introduction

The full text of the article is available for download in PDF format on the right panel.

02 References

  1. 1. Wiik L. Study on Biological and Economic Considerations in the Control of Potato Late Blight and Potato Tuber Blight [Text] / L. Wiik, H. Rosenqvist, E. Liljeroth // J Hortic. – 2018. – V. 5. – P. 226. doi:10.4172/2376-0354.1000226
  2. 2. Haverkort A.J. Societal costs of late blight in potato and prospects of durable resistance through cisgenic modification [Text] / A.J. Haverkort, P.M. Boonekamp, R. Hutten, E. Jacobsen, L.A.P. Lotz // Potato Res. – 2008. – V. 51. – P. 47-57. doi.org/10.1007/s11540-008-9089-y
  3. 3. Haq I. Relative efficacy of various fungicides, chemicals and biochemicals against late blight of potato. Pakistan [Text] / I. Haq, A. Rashid, S.A. Khan // Journal of Phytopathology. – 2008. – V. 21. – P. 129-133. doi/pdf/10.5555/20203248787
  4. 4 Haldar K. Common infection strategies of pathogenic eukaryotes [Text] / K. Haldar, S. Kamoun, N.L. Hiller, S. Bhattacharje, C. van Ooij // Nature Reviews. Microbiology. – 2006. – V. 4. – P. 922-931. doi: 10.1038/nrmicro1549
  5. 5 Ghag S.B. Host induced gene silencing, an emerging science to engineer crop resistance against harmful plant pathogens [Text] / S.B. Ghag // Physiological and Molecular Plant Pathology. – 2017. – V. 100. – 242e254.doi: 10.1016/j.pmpp.2017.10.003
  6. 6 Baum J.A. Progress towards RNAi-mediated insect pest management [Text] / J.A. Baum, J.K. Roberts // Advances in Insect Physiology. – 2014. – V. 47. – P. 249-295. doi: 10.1016/B978-0-12-800197-4.00005-1
  7. 7 Jin H. Endogenous small RNAs and antibacterial immunity in plants [Text] / H. Jin // FEBS Letters. – 2008. – V. 582. – P. 2679-2684. doi:10.1016/j.febslet.2008.06.053
  8. 8 Nowara D. HIGS: Host-Induced Gene Silencing in the obligate biotrophic fungal pathogen Blumeria graminis [Text] / D. Nowara, A. Gay, C. Lacomme, J. Shaw, C. Ridout, D. Douchkov, G. Hensel, J. Kumlehn, P. Schweizer // The Plant Cell. – 2010. – V. 22. – P. 3130-3141. doi: 10.1105/tpc.110.077040
  9. 9 Nizkorodova A.S. Obtaining transgenic potato plants for countering Phytophthora infestans via “HIGS” method implementation [Text] / A.S. Nizkorodova, R.V. Kryldakov, N.Zh. Karimov, N.S. Polimbetova, B.K. Iskakov // Izdenister Natigeler. – 2024 – V. 3(103). – P. 151-158. In english. doi: 10.37884/3-2024/17
  10. 10 Chen X. Plant and animal small RNA communications between cells and organisms [Text] / X. Chen, O. Rechavi // Nat Rev Mol Cell Biol. – 2022– V. 23. – P. 185-203. doi: 10.1038/s41580-021-00425-y
  11. 11 Jahan S.N. Plant-mediated gene silencing restricts growth of the potato late blight pathogen Phytophthora infestans [Text] / S.N. Jahan, A.K.M. Еsman, P. Corcoran, J. Fogelqvist, R.R. Vetukuri, C. Dixelius // Journal of Experimental Botany. – 2015. – V. 66(9). – P. 2785-2794. doi: 10.1093/jxb/erv094
  12. 12 Iuliano A. Introduction to Biostatistics [Text] / A. Iuliano, and M. Franzese // Data from S. Ranganathan, M. Gribskov, K. Nakai, Ch. Schönbach: Encyclopedia of Bioinformatics and Computational Biology, Academic Press, 2019. P. 648-671. doi:10.1016/B978-0-12-809633-8.20353-1
  13. 13 Mohamed A.E.S. Factors affecting in vitro tuberization of potato [Text] / A.ES. Mohamed, N.D. Girgis // Bull Natl Res Cent. – 2023. – V. 47. – P. 80-90. doi: 10.1186/s42269-023-01056-3
  14. 14 Ranalli P. The Canon of Potato Science: 24. Microtubers [Text] / P. Ranalli // Potato Research. – 2007. – V. 50(3). – P. 301-304. doi: 10.1007/s11540-008-9073-6
  15. 15 Karjadi A.K. The effect of light and gibberellic acid concentrations on breaking dormancy of potato micro tuber [Text] / A.K. Karjadi, and N. Waluyo // IOP Conference Series: Earth and Environmental Science. – 2021. – V. 883(1). – P. 12011. doi: 10.1088/1755-1315/883/1/012011
  16. 16 Park W.S. The effect of size and quality of potato microtubers on quality of seed potatoes in the cultivar ‘Superior’ [Text] / W.S. Park, J. Jeon, H.S. Kim, H. Jin, C. Aswath, H. Joung // Scientia Horticulturae. – 2009. – V. 120(1). – P. 127-129. doi: 10.1016/j.scienta.2008.09.00

Citation Links

[1]2025. COMPARATIVE CHARACTERISTICS OF MICROTUBERS OF POTATO VARIETY GALA TRANSGENIC LINES. Izdenister natigeler. 3 (107) (Sep. 2025), 155–165. DOI:https://doi.org/10.37884/3-2025/14.
(1)COMPARATIVE CHARACTERISTICS OF MICROTUBERS OF POTATO VARIETY GALA TRANSGENIC LINES. Izdenister natigeler 2025, No. 3 (107), 155-165. https://doi.org/10.37884/3-2025/14.
COMPARATIVE CHARACTERISTICS OF MICROTUBERS OF POTATO VARIETY GALA TRANSGENIC LINES. (2025). Izdenister Natigeler, 3 (107), 155-165. https://doi.org/10.37884/3-2025/14
COMPARATIVE CHARACTERISTICS OF MICROTUBERS OF POTATO VARIETY GALA TRANSGENIC LINES. Izdenister natigeler, [S. l.], n. 3 (107), p. 155–165, 2025. DOI: 10.37884/3-2025/14. Disponível em: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/968. Acesso em: 15 sep. 2026.
“COMPARATIVE CHARACTERISTICS OF MICROTUBERS OF POTATO VARIETY GALA TRANSGENIC LINES”. 2025. Izdenister Natigeler, no. 3 (107) (September): 155-65. https://doi.org/10.37884/3-2025/14.
“COMPARATIVE CHARACTERISTICS OF MICROTUBERS OF POTATO VARIETY GALA TRANSGENIC LINES” (2025) Izdenister natigeler, (3 (107), pp. 155–165. doi:10.37884/3-2025/14.
[1]“COMPARATIVE CHARACTERISTICS OF MICROTUBERS OF POTATO VARIETY GALA TRANSGENIC LINES”, Izdenister natigeler, no. 3 (107), pp. 155–165, Sep. 2025, doi: 10.37884/3-2025/14.
“COMPARATIVE CHARACTERISTICS OF MICROTUBERS OF POTATO VARIETY GALA TRANSGENIC LINES”. Izdenister Natigeler, no. 3 (107), Sept. 2025, pp. 155-6, https://doi.org/10.37884/3-2025/14.
“COMPARATIVE CHARACTERISTICS OF MICROTUBERS OF POTATO VARIETY GALA TRANSGENIC LINES”. Izdenister natigeler, no. 3 (107) (September 30, 2025): 155–165. Accessed September 15, 2026. https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/968.
1.COMPARATIVE CHARACTERISTICS OF MICROTUBERS OF POTATO VARIETY GALA TRANSGENIC LINES. Izdenister natigeler [Internet]. 2025 Sep. 30 [cited 2026 Sep. 15];(3 (107):155-6. Available from: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/968
1.COMPARATIVE CHARACTERISTICS OF MICROTUBERS OF POTATO VARIETY GALA TRANSGENIC LINES. Izdenister natigeler. 2025;(3 (107):155-165. doi:10.37884/3-2025/14