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STOCK-RAISING AND VETERINARY

INVESTIGATION OF CAMEL MILK WHEY PROTEINS BY SDS-PAGE METHOD

Kuanysh Syman 1 , Aray Kirgizbayeva 2 , Zhansaya Abdukadirova 3 , Roza Bakessova 4 , Ayagoz Mendigaliyeva 4

1 Abai Kazakh National Pedagogical University, Almaty, Kazakhstan; 2 Kazakh National Medical University named after S.D. Asfendiyarova, Almaty, Kazakhstan; 3 Al-Farabi Kazakh National University, Almaty, Kazakhstan; 4 West Kazakhstan Innovative and Technological University, Uralsk, Kazakhstan

doi.org/10.37884/3-2026/04 pp. 51-61 Admitted 23.04.2026 Published 30.06.2026

Abstract

Camel milk is a traditional and strategically important product for the desert regions of Ka
zakhstan. Its therapeutic and prophylactic properties are associated with whey protein composition. However,
the SDS-PAGE profile of whey proteins from Bactrian and dromedary camels under Kazakhstan conditions
has not been studied. The aim was to determine the SDS-PAGE profile of whey proteins from Bactrian,
dromedary, and bulk camel milk, to assess their quantitative composition, electrophoretic mobility, and spe
cies-specific differences. Whey proteins were isolated by isoelectric precipitation at pH 4.6 and centrifugation
at 10000 × g. Electrophoresis was performed in 12 % polyacrylamide gel with SDS according to Laemmli. Six
standard proteins were used as markers: 94, 67, 43, 30, 20, and 14.4 kDa. Relative content of bands was
determined by densitometric analysis using the GelDoc XR+ system. 9 whey protein bands were detected in
Bactrian and bulk milk, 8 in dromedary milk. Dominant fractions: serum albumin 20.0–21.2 % Rf=0.15–0.18
and immunoglobulins 19.4–19.8 % Rf=0.09–0.11. The proportion of α-lactalbumin was 9.7–10.1 % Rf=0.92
0.93. Lactoferrin ∼
55 kDa Rf=0.46–0.48 was identified, with a content of 8.3–9.3 %. β-Lactoglobulin, char
acteristic of cow milk, was absent in all camel milk samples. The key species difference is the absence of band
7 in the dromedary. This band is present in both Bactrian and bulk milk. Fraction #4 in dromedary had a
significantly higher content: 12.2 ± 0.5 % vs 8.1 ± 0.3 % in Bactrian. The SDS-PAGE profile of whey proteins
from Bactrian and dromedary camel milk was established for the first time under Kazakhstan conditions. The
absence of β-lactoglobulin confirms the hypoallergenic properties of camel milk at the molecular level. The
high proportion of immunoglobulins and serum albumin indicates its biological value and provides a basis for
developing functional foods.

camel milk, whey proteins, SDS-PAGE, albumin, immunoglobulins, β-lactoglobulin, hy poallergenic, bactrian, dromedary

01 Introduction

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02 References

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Citation Links

[1]2026. INVESTIGATION OF CAMEL MILK WHEY PROTEINS BY SDS-PAGE METHOD. Izdenister natigeler. 28, 3 (111) (Jun. 2026), 51–61. DOI:https://doi.org/10.37884/3-2026/04.
(1)INVESTIGATION OF CAMEL MILK WHEY PROTEINS BY SDS-PAGE METHOD. Izdenister natigeler 2026, 28 (3 (111), 51-61. https://doi.org/10.37884/3-2026/04.
INVESTIGATION OF CAMEL MILK WHEY PROTEINS BY SDS-PAGE METHOD. (2026). Izdenister Natigeler, 28(3 (111), 51-61. https://doi.org/10.37884/3-2026/04
INVESTIGATION OF CAMEL MILK WHEY PROTEINS BY SDS-PAGE METHOD. Izdenister natigeler, [S. l.], v. 28, n. 3 (111), p. 51–61, 2026. DOI: 10.37884/3-2026/04. Disponível em: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/1358. Acesso em: 15 sep. 2026.
“ INVESTIGATION OF CAMEL MILK WHEY PROTEINS BY SDS-PAGE METHOD”. 2026. Izdenister Natigeler 28 (3 (111): 51-61. https://doi.org/10.37884/3-2026/04.
“ INVESTIGATION OF CAMEL MILK WHEY PROTEINS BY SDS-PAGE METHOD” (2026) Izdenister natigeler, 28(3 (111), pp. 51–61. doi:10.37884/3-2026/04.
[1]“ INVESTIGATION OF CAMEL MILK WHEY PROTEINS BY SDS-PAGE METHOD”, Izdenister natigeler, vol. 28, no. 3 (111), pp. 51–61, Jun. 2026, doi: 10.37884/3-2026/04.
“ INVESTIGATION OF CAMEL MILK WHEY PROTEINS BY SDS-PAGE METHOD”. Izdenister Natigeler, vol. 28, no. 3 (111), June 2026, pp. 51-61, https://doi.org/10.37884/3-2026/04.
“ INVESTIGATION OF CAMEL MILK WHEY PROTEINS BY SDS-PAGE METHOD”. Izdenister natigeler 28, no. 3 (111) (June 30, 2026): 51–61. Accessed September 15, 2026. https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/1358.
1.INVESTIGATION OF CAMEL MILK WHEY PROTEINS BY SDS-PAGE METHOD. Izdenister natigeler [Internet]. 2026 Jun. 30 [cited 2026 Sep. 15];28(3 (111):51-6. Available from: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/1358
1.INVESTIGATION OF CAMEL MILK WHEY PROTEINS BY SDS-PAGE METHOD. Izdenister natigeler. 2026;28(3 (111):51-61. doi:10.37884/3-2026/04