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AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

OPTIMIZATION OF THE NUTRIENT MEDIUM COMPOSITION FOR IN VITRO MICROPROPAGATION OF PEAR ROOTSTOCKS

Zarina Yussupova 1 , Toigul Nurseitova 2 , Irina Kovalchuk 3 , Balnur Kabylbekova 2

1 Kazakh Fruit and Vegetable Research Institute, Kazakh National Agrarian Research University, Almaty, Kazakhstan; 2 Kazakh Fruit and Vegetable Research Institute, Almaty, Kazakhstan; 3 Kazakh Fruit and Vegetable Research Institute, Almaty, Kazakhstan;

doi.org/10.37884/1-2026/12 pp. 115-123 Admitted 06.01.2026 Published 27.02.2026

Abstract

Introduction. The significant reduction of orchards in recent decades is associated with the impact of unfavorable abiotic factors and the spread of bacterial pathogens, leading to a considerable decline in pear cultivar diversity. The rapid introduction of resistant cultivars and rootstocks into commercial horticulture is constrained by the limited availability of high-quality planting material. Methods. This study presents the results of clonal micropropagation of the seedling pear rootstock Pyrus pyraster and clonal rootstocks OHxF230 and OHxF69 . An optimal explant sterilization protocol ensuring high survival during the establishment of aseptic culture was developed. The effects of different concentrations of nitrogen salts (NH₄NO₃ and KNO₃) and plant growth regulators (BAP, IBA, GA₃) on shoot proliferation were evaluated. Results. Sterilization of Pyrus pyraster explants with 0.2 % HgCl₂ for 4 min resulted in the highest shoot regeneration rate (78.57 %), whereas shorter exposure increased contamination levels. The highest multiplication efficiency was achieved on a medium containing 75 % of the standard nitrate salt concentration supplemented with 0.8 mg L⁻¹ BAP, 0.5 mg L⁻¹ GA₃, and 0.1 mg L⁻¹ IBA. In contrast, a higher BAP concentration (1.8 mg L⁻¹) combined with reduced nitrogen levels decreased proliferation efficiency, particularly in Pyrus pyraster. However, a relatively high multiplication coefficient (2.67 ± 1.15) was observed for OHxF230 . Conclusions. The results demonstrate the potential to optimize in vitro culture conditions to enhance the efficiency of pear rootstock micropropagation and can be applied to the development of biotechnological propagation protocols.

pear rootstock Pyrus pyraster micropropagation nutrient medium sterilization in vitro

01 Introduction

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02 References

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Citation Links

[1]2026. OPTIMIZATION OF THE NUTRIENT MEDIUM COMPOSITION FOR IN VITRO MICROPROPAGATION OF PEAR ROOTSTOCKS. Izdenister natigeler. 28, 1(109) (Feb. 2026), 115–123. DOI:https://doi.org/10.37884/1-2026/12.
(1)OPTIMIZATION OF THE NUTRIENT MEDIUM COMPOSITION FOR IN VITRO MICROPROPAGATION OF PEAR ROOTSTOCKS. Izdenister natigeler 2026, 28 (1(109), 115-123. https://doi.org/10.37884/1-2026/12.
OPTIMIZATION OF THE NUTRIENT MEDIUM COMPOSITION FOR IN VITRO MICROPROPAGATION OF PEAR ROOTSTOCKS. (2026). Izdenister Natigeler, 28(1(109), 115-123. https://doi.org/10.37884/1-2026/12
OPTIMIZATION OF THE NUTRIENT MEDIUM COMPOSITION FOR IN VITRO MICROPROPAGATION OF PEAR ROOTSTOCKS. Izdenister natigeler, [S. l.], v. 28, n. 1(109), p. 115–123, 2026. DOI: 10.37884/1-2026/12. Disponível em: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/1154. Acesso em: 15 sep. 2026.
“OPTIMIZATION OF THE NUTRIENT MEDIUM COMPOSITION FOR IN VITRO MICROPROPAGATION OF PEAR ROOTSTOCKS”. 2026. Izdenister Natigeler 28 (1(109): 115-23. https://doi.org/10.37884/1-2026/12.
“OPTIMIZATION OF THE NUTRIENT MEDIUM COMPOSITION FOR IN VITRO MICROPROPAGATION OF PEAR ROOTSTOCKS” (2026) Izdenister natigeler, 28(1(109), pp. 115–123. doi:10.37884/1-2026/12.
[1]“OPTIMIZATION OF THE NUTRIENT MEDIUM COMPOSITION FOR IN VITRO MICROPROPAGATION OF PEAR ROOTSTOCKS”, Izdenister natigeler, vol. 28, no. 1(109), pp. 115–123, Feb. 2026, doi: 10.37884/1-2026/12.
“OPTIMIZATION OF THE NUTRIENT MEDIUM COMPOSITION FOR IN VITRO MICROPROPAGATION OF PEAR ROOTSTOCKS”. Izdenister Natigeler, vol. 28, no. 1(109), Feb. 2026, pp. 115-23, https://doi.org/10.37884/1-2026/12.
“OPTIMIZATION OF THE NUTRIENT MEDIUM COMPOSITION FOR IN VITRO MICROPROPAGATION OF PEAR ROOTSTOCKS”. Izdenister natigeler 28, no. 1(109) (February 27, 2026): 115–123. Accessed September 15, 2026. https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/1154.
1.OPTIMIZATION OF THE NUTRIENT MEDIUM COMPOSITION FOR IN VITRO MICROPROPAGATION OF PEAR ROOTSTOCKS. Izdenister natigeler [Internet]. 2026 Feb. 27 [cited 2026 Sep. 15];28(1(109):115-23. Available from: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/1154
1.OPTIMIZATION OF THE NUTRIENT MEDIUM COMPOSITION FOR IN VITRO MICROPROPAGATION OF PEAR ROOTSTOCKS. Izdenister natigeler. 2026;28(1(109):115-123. doi:10.37884/1-2026/12