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STOCK-RAISING AND VETERINARY

DETECTION OF IGFBP3 GENE POLYMORPHISM AND PHYLOGENETIC ANALYSIS IN KAZAKH FAT-TAILED COARSE-WOOL SHEEP BREED

Marina Yermekova 1 , Zulkharnay Bekhtasov 1 , Altynay Kozhakhmet 2 , Kairat Dossybayev 1 , Tіlek Kapasuly 2 , Rauan Amzeev 3 , Makpal Amandykova 2

1 LLP “Kazakh Research Institute of Livestock and Foraging”; 2 Institute of Genetics and Physiology (RSE, CS MES RK); 3 “Gymnasium No. 159 named after Y. Altynsarin”

doi.org/10.37884/3-2025/05 pp. 42-54 Admitted 16.07.2025 Published 30.09.2025

Abstract

This study aims to investigate the polymorphism of the IGFBP3 gene in the Kazakh coarse wool sheep breed. PCR-RFLP and Sanger sequencing methods were applied in the research. Genomic DNA was extracted from peripheral blood samples of 380 sheep, and its quality and concentration were determined by spectrophotometry and agarose gel electrophoresis. PCR and Hae III restriction enzyme treatment were used to identify the polymorphisms of the IGFBP3 gene, resulting in the identification of 6 potential polymorphisms. Sanger sequencing revealed a C>G substitution at position 335 and a missense mutation caused by the Ala>Gly amino acid substitution at position 111. Based on the obtained data, phylogenetic analysis of the Kazakh coarse wool sheep was performed with other sheep breeds available in the NCBI database. The phylogenetic tree consisted of two clusters. This study, utilizing IGFBP3 gene polymorphisms, contributes to improving breeding programs in sheep farming.

The results of this study scientifically confirmed, for the first time, the presence of genetic markers potentially influencing productivity in the Kazakh fat-tailed coarse-wool sheep breed. The identified missense mutation in the IGFBP3 gene provides new opportunities for selecting economically valuable traits in breeding programs. Phylogenetic analysis of the obtained sequences revealed genetic diversity and relationships among sheep breeds. These findings are crucial for the effective utilization of genetic resources and the preservation of adaptive traits of local sheep populations. Verification of the results through comparison with international scientific databases ensures their reliability and high applied significance.

IGFBP3 gene polymorphism PCR Sanger method sequencing heterozygous genotype phylogenetic analysis Kazakh coarse wool sheep genetic markers sheep breed

01 Introduction

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02 References

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Citation Links

[1]2025. DETECTION OF IGFBP3 GENE POLYMORPHISM AND PHYLOGENETIC ANALYSIS IN KAZAKH FAT-TAILED COARSE-WOOL SHEEP BREED. Izdenister natigeler. 3 (107) (Sep. 2025), 42–54. DOI:https://doi.org/10.37884/3-2025/05.
(1)DETECTION OF IGFBP3 GENE POLYMORPHISM AND PHYLOGENETIC ANALYSIS IN KAZAKH FAT-TAILED COARSE-WOOL SHEEP BREED. Izdenister natigeler 2025, No. 3 (107), 42-54. https://doi.org/10.37884/3-2025/05.
DETECTION OF IGFBP3 GENE POLYMORPHISM AND PHYLOGENETIC ANALYSIS IN KAZAKH FAT-TAILED COARSE-WOOL SHEEP BREED. (2025). Izdenister Natigeler, 3 (107), 42-54. https://doi.org/10.37884/3-2025/05
DETECTION OF IGFBP3 GENE POLYMORPHISM AND PHYLOGENETIC ANALYSIS IN KAZAKH FAT-TAILED COARSE-WOOL SHEEP BREED. Izdenister natigeler, [S. l.], n. 3 (107), p. 42–54, 2025. DOI: 10.37884/3-2025/05. Disponível em: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/1038. Acesso em: 15 sep. 2026.
“DETECTION OF IGFBP3 GENE POLYMORPHISM AND PHYLOGENETIC ANALYSIS IN KAZAKH FAT-TAILED COARSE-WOOL SHEEP BREED”. 2025. Izdenister Natigeler, no. 3 (107) (September): 42-54. https://doi.org/10.37884/3-2025/05.
“DETECTION OF IGFBP3 GENE POLYMORPHISM AND PHYLOGENETIC ANALYSIS IN KAZAKH FAT-TAILED COARSE-WOOL SHEEP BREED” (2025) Izdenister natigeler, (3 (107), pp. 42–54. doi:10.37884/3-2025/05.
[1]“DETECTION OF IGFBP3 GENE POLYMORPHISM AND PHYLOGENETIC ANALYSIS IN KAZAKH FAT-TAILED COARSE-WOOL SHEEP BREED”, Izdenister natigeler, no. 3 (107), pp. 42–54, Sep. 2025, doi: 10.37884/3-2025/05.
“DETECTION OF IGFBP3 GENE POLYMORPHISM AND PHYLOGENETIC ANALYSIS IN KAZAKH FAT-TAILED COARSE-WOOL SHEEP BREED”. Izdenister Natigeler, no. 3 (107), Sept. 2025, pp. 42-54, https://doi.org/10.37884/3-2025/05.
“DETECTION OF IGFBP3 GENE POLYMORPHISM AND PHYLOGENETIC ANALYSIS IN KAZAKH FAT-TAILED COARSE-WOOL SHEEP BREED”. Izdenister natigeler, no. 3 (107) (September 30, 2025): 42–54. Accessed September 15, 2026. https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/1038.
1.DETECTION OF IGFBP3 GENE POLYMORPHISM AND PHYLOGENETIC ANALYSIS IN KAZAKH FAT-TAILED COARSE-WOOL SHEEP BREED. Izdenister natigeler [Internet]. 2025 Sep. 30 [cited 2026 Sep. 15];(3 (107):42-54. Available from: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/1038
1.DETECTION OF IGFBP3 GENE POLYMORPHISM AND PHYLOGENETIC ANALYSIS IN KAZAKH FAT-TAILED COARSE-WOOL SHEEP BREED. Izdenister natigeler. 2025;(3 (107):42-54. doi:10.37884/3-2025/05