ОЗДОРОВЛЕНИЕ И РАЗМНОЖЕНИЕ КЛОНОВЫХ ПОДВОЕВ И СОРТОВ СЛИВЫ И ВИШНИ В КУЛЬТУРЕ ТКАНЕЙ В СОЧЕТАНИИ С ТЕРМОТЕРАПИЕЙ

Светлана Долгих; Молдир Саршаева; Акерке Ташкенбаева

doi:10.37884/3-2025/11

Abstract

Viral diseases cause significant harm to fruit crops, often making it economically unprofitable to operate plantations or causing the death of plants in the nursery and gardens. The main thing in the prevention of viral infections is the planting of industrial plantations with cured planting material. The production of improved planting material consists of several stages. Initial plants choose plants, typical for the variety, characterized by high productivity. After the selection of the original plants, they are tested for viral infection and recovery (if testing revealed diseases) and reproduction. Healing of the initial plants is carried out by the method of apical meristem culture, thermotherapy methods, chemotherapy and their combination. The article presents data on the diagnosis of juice-carrying viruses by the method of herbaceous indicators and RT-PCR, the improvement of varieties and rootstocks of plums and cherries by the method of culture of apical meristem in combination with thermotherapy. The stages of clonal micropropagation technology from sterilization of apex tissues introduced into the culture, regeneration, proliferation (reproduction) and rhizogenesis (rooting in vitro) have been worked out. The influence of monochromatic light on the processes of regeneration of plant tissue is shown in vitro. In culture in vitro introduced plants two varieties and two rootstocks of plum, two varieties and two rootstocks of cherry. For each varieties and rootstocks optimized conditions for sterilization of exploiters introduction to tissue culture. The composition of nutrients in stages introduction in vitro, micropropagation and rooting microcloned plants. Thermotherapy regimen worked out. Established by positive effect of red and blue LED lamps on the proliferation of apex varieties and rootstocks of stone crops. In culture ex vitro obtained virus-free pre-basic uterine plants of clone rootstocks WC-13 and BBA-1.

Cherry plum virus diagnostics apical meristem culture thermotherapy clonal micropropagation monochromatic light adaptation ex vitro

01 Introduction

The full text of the article is available for download in PDF format on the right panel.

02 References

1 Борисова А. А. РОЛЬ И ЗНАЧЕНИЕ СЕРТИФИКАЦИИ ПОСАДОЧНОГО МАТЕРИАЛА В САДОВОДСТВЕ РОССИИ //САДОВОДСТВО И ВИНОГРАДАРСТВО. – 2010. – №. 4. – С. 22-24.
2 Уразаева М., Ефремова Ю., Ормакаев А. ИНТРОДУЦИРОВАННЫЕ КЛОНОВЫЕ ПОДВОИ КОСТОЧКОВЫХ //Izdenister natigeler. – 2023. – №. 2 (98). – С. 143-153. https://doi.org/10.37884/2-2023/14
3 Кабылбекова Б., Нурсейтова Т., Юсупова З., Ковальчук И., Чуканова Н., Уразаева М., Маденова А. ОПРЕДЕЛЕНИЕ СОСТАВА ВИРУСНЫХ БОЛЕЗНЕЙ КЛОНОВЫХ ПОДВОЕВ КОСТОЧКОВЫХ КУЛЬТУР В КАЗАХСТАНЕ И ОСОБЕННОСТИ РАЗМНОЖЕНИЯ IN VITRO ЗДОРОВЫХ РАСТЕНИЙ//Izdenister natigeler. – 2024. – №. 3 (103). – С. 86-97.https://doi.org/10.37884/3-2024/10
4 Минаев В. А., Верзилин А. В., Высоцкий В. А. ЭФФЕКТИВНОСТЬ КЛОНАЛЬНОГО МИКРОРАЗМНОЖЕНИЯ И КАЧЕСТВО ОЗДОРОВЛЕННОГО ПОДВОЙНОГО МАТЕРИАЛА ЯБЛОНИ //Садоводство и виноградарство. – 2005. – №. 4. – С. 12-14.
5 Кухарчик Н.В., Кастрицкая М.С., Колбанова Е.В. и др. РАЗМНОЖЕНИЕ ПЛОДОВЫХ, ЯГОДНЫХ РАСТЕНИЙ, ВИНОГРАДА И ХМЕЛЯ В КУЛЬТУРЕ IN VITRO.-Минск.- изд. «Колорград».- 2021.-397 с.
6 Min-Rui Wang, Zhen-Hua Cui, Jing-Wei Li, Xin-Yi Hao &Qiao-Chun Wang. IN VITRO THERMOTHERAPY- BASED METHODS FOR PLANT VIRUS ERADICATION//Plant Methods 14, 87.-2018
7 Barba M, Ilardi V, Pasquini G. CONTROL OF POME AND STONE FRUIT VIRUS DISEASES. In: Loebenstein G, Katis NI, editors. Advances in virus research, vol.91. Burling ton: Academic Press, 2015. P.47-83
8 EPPO Standards - Certification schemes -PM 4/27(1) Pathogen-tested material of Malus, Pyrus and Cydonia // EPPO Bulletin. - 1999. -Vol. 29, № 3. - P. 239-252.
9 Лукичева Л. А., Митрофанова О. В., Лесникова-Седошенко Н. П. ОЗДОРОВЛЕНИЕ СОРТОВ ВИШНИ (PRUNUS CERASUS L.) И СЛИВЫ (PRUNUS DOMESTICA L.) ОТ ВИРУСОВ С ИСПОЛЬЗОВАНИЕМ БИОТЕХНОЛОГИЧЕСКИХ ПРИЕМОВ //Биология растений и садоводство: теория, инновации. – 2007. – №. 127. – С. 27-34.
10 Долгих С.Г. Рекомендации «ТЕХНОЛОГИЯ ПРОИЗВОДСТВА БЕЗВИРУСНОГО ПОСАДОЧНОГО МАТЕРИАЛА ПЛОДОВЫХ, ЯГОДНЫХ КУЛЬТУР И ВИНОГРАДА».- 2020.- 57 с.
11 Упадышев М. Т. и др. ДИАГНОСТИКА ВИРУСОВ СЕМЕЧКОВЫХ И КОСТОЧКОВЫХ КУЛЬТУР МЕТОДАМИ ИФА И ПЦР. – 2008.
12 Rwahnih M. A. et al. MOLECULAR VARIABILITY OF APPLE CHLOROTIC LEAF SPOT VIRUSIN DIFFERENT HOSTS AND GEOGRAPHICAL REGIONS //Journal of Plant Pathology. – 2004. – С. 117-122.
13 Murashige T., Skoog F. A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES //Physiologia plantarum. – 1962. – Т. 15. – №. 3.
14 Nitsch J. P., Nitsch C. HAPLOID PLANTS FROM POLLEN GRAINS //Science. – 1969. – Т. 163. – №. 3862. – С. 85-87.
15 White P. R. THE CULTIVATION OF ANIMAL AND PLANT CELLS //(No Title). – 1963.
16 Gamborg O. L., Miller R. A., Ojima K. NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS //Experimental cell research. – 1968. – Т. 50. – №. 1. – С. 151-158.
17 Anderson W. C. ROOTING OF TISSUE CULTURED RHODODENDRONS. – 1978.
18 Lloyd G., McCown B. COMMERCIALLY-FEASIBLE MICROPROPAGATION OF MOUNTAIN LAUREL, KALMIA LATIFOLIA, BY USE OF SHOOT-TIP CULTURE. – 1980.
19 Quoirin M., Lepoivre P. H. ImprOVED MEDIA FOR IN VITRO CULTURE OF PRUNUS SPECIES //Symposium on Tissue Culture for Horticultural Purposes 78. – 1977. – С. 437-442.

Citation Links

[1]2025. HEALING AND PROPAGATION OF CLONAL ROOTSTOCKS AND VARIETIES OF PLUM AND CHERRIES IN TISSUE CULTURE IN COMBINATION WITH THERMOTHERAPY. Izdenister natigeler. 3 (107) (Sep. 2025), 123–135. DOI:https://doi.org/10.37884/3-2025/11.

(1)HEALING AND PROPAGATION OF CLONAL ROOTSTOCKS AND VARIETIES OF PLUM AND CHERRIES IN TISSUE CULTURE IN COMBINATION WITH THERMOTHERAPY. Izdenister natigeler 2025, No. 3 (107), 123-135. https://doi.org/10.37884/3-2025/11.

HEALING AND PROPAGATION OF CLONAL ROOTSTOCKS AND VARIETIES OF PLUM AND CHERRIES IN TISSUE CULTURE IN COMBINATION WITH THERMOTHERAPY. (2025). Izdenister Natigeler, 3 (107), 123-135. https://doi.org/10.37884/3-2025/11

HEALING AND PROPAGATION OF CLONAL ROOTSTOCKS AND VARIETIES OF PLUM AND CHERRIES IN TISSUE CULTURE IN COMBINATION WITH THERMOTHERAPY. Izdenister natigeler, [S. l.], n. 3 (107), p. 123–135, 2025. DOI: 10.37884/3-2025/11. Disponível em: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/963. Acesso em: 15 sep. 2026.

“HEALING AND PROPAGATION OF CLONAL ROOTSTOCKS AND VARIETIES OF PLUM AND CHERRIES IN TISSUE CULTURE IN COMBINATION WITH THERMOTHERAPY”. 2025. Izdenister Natigeler, no. 3 (107) (September): 123-35. https://doi.org/10.37884/3-2025/11.

“HEALING AND PROPAGATION OF CLONAL ROOTSTOCKS AND VARIETIES OF PLUM AND CHERRIES IN TISSUE CULTURE IN COMBINATION WITH THERMOTHERAPY” (2025) Izdenister natigeler, (3 (107), pp. 123–135. doi:10.37884/3-2025/11.

[1]“HEALING AND PROPAGATION OF CLONAL ROOTSTOCKS AND VARIETIES OF PLUM AND CHERRIES IN TISSUE CULTURE IN COMBINATION WITH THERMOTHERAPY”, Izdenister natigeler, no. 3 (107), pp. 123–135, Sep. 2025, doi: 10.37884/3-2025/11.

“HEALING AND PROPAGATION OF CLONAL ROOTSTOCKS AND VARIETIES OF PLUM AND CHERRIES IN TISSUE CULTURE IN COMBINATION WITH THERMOTHERAPY”. Izdenister Natigeler, no. 3 (107), Sept. 2025, pp. 123-35, https://doi.org/10.37884/3-2025/11.

“HEALING AND PROPAGATION OF CLONAL ROOTSTOCKS AND VARIETIES OF PLUM AND CHERRIES IN TISSUE CULTURE IN COMBINATION WITH THERMOTHERAPY”. Izdenister natigeler, no. 3 (107) (September 30, 2025): 123–135. Accessed September 15, 2026. https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/963.

1.HEALING AND PROPAGATION OF CLONAL ROOTSTOCKS AND VARIETIES OF PLUM AND CHERRIES IN TISSUE CULTURE IN COMBINATION WITH THERMOTHERAPY. Izdenister natigeler [Internet]. 2025 Sep. 30 [cited 2026 Sep. 15];(3 (107):123-35. Available from: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/963

1.HEALING AND PROPAGATION OF CLONAL ROOTSTOCKS AND VARIETIES OF PLUM AND CHERRIES IN TISSUE CULTURE IN COMBINATION WITH THERMOTHERAPY. Izdenister natigeler. 2025;(3 (107):123-135. doi:10.37884/3-2025/11

[1] 1 Борисова А. А. РОЛЬ И ЗНАЧЕНИЕ СЕРТИФИКАЦИИ ПОСАДОЧНОГО МАТЕРИАЛА В САДОВОДСТВЕ РОССИИ //САДОВОДСТВО И ВИНОГРАДАРСТВО. – 2010. – №. 4. – С. 22-24.

[2] 2 Уразаева М., Ефремова Ю., Ормакаев А. ИНТРОДУЦИРОВАННЫЕ КЛОНОВЫЕ ПОДВОИ КОСТОЧКОВЫХ //Izdenister natigeler. – 2023. – №. 2 (98). – С. 143-153. https://doi.org/10.37884/2-2023/14

[3] 3 Кабылбекова Б., Нурсейтова Т., Юсупова З., Ковальчук И., Чуканова Н., Уразаева М., Маденова А. ОПРЕДЕЛЕНИЕ СОСТАВА ВИРУСНЫХ БОЛЕЗНЕЙ КЛОНОВЫХ ПОДВОЕВ КОСТОЧКОВЫХ КУЛЬТУР В КАЗАХСТАНЕ И ОСОБЕННОСТИ РАЗМНОЖЕНИЯ IN VITRO ЗДОРОВЫХ РАСТЕНИЙ//Izdenister natigeler. – 2024. – №. 3 (103). – С. 86-97.https://doi.org/10.37884/3-2024/10

[4] 4 Минаев В. А., Верзилин А. В., Высоцкий В. А. ЭФФЕКТИВНОСТЬ КЛОНАЛЬНОГО МИКРОРАЗМНОЖЕНИЯ И КАЧЕСТВО ОЗДОРОВЛЕННОГО ПОДВОЙНОГО МАТЕРИАЛА ЯБЛОНИ //Садоводство и виноградарство. – 2005. – №. 4. – С. 12-14.

[5] 5 Кухарчик Н.В., Кастрицкая М.С., Колбанова Е.В. и др. РАЗМНОЖЕНИЕ ПЛОДОВЫХ, ЯГОДНЫХ РАСТЕНИЙ, ВИНОГРАДА И ХМЕЛЯ В КУЛЬТУРЕ IN VITRO.-Минск.- изд. «Колорград».- 2021.-397 с.

[6] 6 Min-Rui Wang, Zhen-Hua Cui, Jing-Wei Li, Xin-Yi Hao &Qiao-Chun Wang. IN VITRO THERMOTHERAPY- BASED METHODS FOR PLANT VIRUS ERADICATION//Plant Methods 14, 87.-2018

[7] 7 Barba M, Ilardi V, Pasquini G. CONTROL OF POME AND STONE FRUIT VIRUS DISEASES. In: Loebenstein G, Katis NI, editors. Advances in virus research, vol.91. Burling ton: Academic Press, 2015. P.47-83

[8] 8 EPPO Standards - Certification schemes -PM 4/27(1) Pathogen-tested material of Malus, Pyrus and Cydonia // EPPO Bulletin. - 1999. -Vol. 29, № 3. - P. 239-252.

[9] 9 Лукичева Л. А., Митрофанова О. В., Лесникова-Седошенко Н. П. ОЗДОРОВЛЕНИЕ СОРТОВ ВИШНИ (PRUNUS CERASUS L.) И СЛИВЫ (PRUNUS DOMESTICA L.) ОТ ВИРУСОВ С ИСПОЛЬЗОВАНИЕМ БИОТЕХНОЛОГИЧЕСКИХ ПРИЕМОВ //Биология растений и садоводство: теория, инновации. – 2007. – №. 127. – С. 27-34.

[10] 10 Долгих С.Г. Рекомендации «ТЕХНОЛОГИЯ ПРОИЗВОДСТВА БЕЗВИРУСНОГО ПОСАДОЧНОГО МАТЕРИАЛА ПЛОДОВЫХ, ЯГОДНЫХ КУЛЬТУР И ВИНОГРАДА».- 2020.- 57 с.

[11] 11 Упадышев М. Т. и др. ДИАГНОСТИКА ВИРУСОВ СЕМЕЧКОВЫХ И КОСТОЧКОВЫХ КУЛЬТУР МЕТОДАМИ ИФА И ПЦР. – 2008.

[12] 12 Rwahnih M. A. et al. MOLECULAR VARIABILITY OF APPLE CHLOROTIC LEAF SPOT VIRUSIN DIFFERENT HOSTS AND GEOGRAPHICAL REGIONS //Journal of Plant Pathology. – 2004. – С. 117-122.

[13] 13 Murashige T., Skoog F. A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES //Physiologia plantarum. – 1962. – Т. 15. – №. 3.

[14] 14 Nitsch J. P., Nitsch C. HAPLOID PLANTS FROM POLLEN GRAINS //Science. – 1969. – Т. 163. – №. 3862. – С. 85-87.

[15] 15 White P. R. THE CULTIVATION OF ANIMAL AND PLANT CELLS //(No Title). – 1963.

[16] 16 Gamborg O. L., Miller R. A., Ojima K. NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS //Experimental cell research. – 1968. – Т. 50. – №. 1. – С. 151-158.

[17] 17 Anderson W. C. ROOTING OF TISSUE CULTURED RHODODENDRONS. – 1978.

[18] 18 Lloyd G., McCown B. COMMERCIALLY-FEASIBLE MICROPROPAGATION OF MOUNTAIN LAUREL, KALMIA LATIFOLIA, BY USE OF SHOOT-TIP CULTURE. – 1980.

[19] 19 Quoirin M., Lepoivre P. H. ImprOVED MEDIA FOR IN VITRO CULTURE OF PRUNUS SPECIES //Symposium on Tissue Culture for Horticultural Purposes 78. – 1977. – С. 437-442.