ОПРЕДЕЛЕНИЕ СОСТАВА ВИРУСНЫХ БОЛЕЗНЕЙ КЛОНОВЫХ ПОДВОЕВ КОСТОЧКОВЫХ КУЛЬТУР В КАЗАХСТАНЕ И ОСОБЕННОСТИ РАЗМНОЖЕНИЯ IN VITRO ЗДОРОВЫХ РАСТЕНИЙ

Балнур Кабылбекова; Тойгул Нурсейтова; Зарина Юсупова; Ирина Ковальчук; Нина Чуканова; Марина Уразаева; Айгуль Маденова

doi:10.37884/3-2024/10

Abstract

One of the important tasks of nursery farming in Kazakhstan is the need to produce not only standard, but also virus-free certified planting material. The first step in this direction with the aim of re-equipping horticulture in the republic is the planting of basic mother plantations with virus-free planting material. The solution of these issues became possible with the development and implementation of biotechnological systems in nursery farming. The article presents the composition of viral diseases of stone fruit clonal rootstocks in Kazakhstan and the results of improving the protocol of their clonal micropropagation at various stages. A collection of clonal rootstocks for plum, apricot and peach, consisting of 9 genotypes, was tested for the presence of 7 viruses. According to the result of PCR analysis, clonal rootstocks are free of viral diseases, and the Plum pox potyvirus (PPV) virus was detected in form No. 3 of the wild apricot P. armeniaca.

Virus-free plants were introduced into aseptic culture in vitro. The experiments showed that the shoots of stone fruits rootstocks are effectively sterilized from saprophytic microflora with 0.2% HgCl2 for 3 and 4 minutes. In this case, the number of those capable of regeneration is 70% and 80%.

clonal rootstocks, stone fruits, viruses, biotechnology, in vitro propagation, nutrient medium, virus-free material.

01 Introduction

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02 References

1 Panis, B., Nagel, M., & Van den Houwe, I. (2020). Challenges and prospects for the conservation of crop genetic resources in field genebanks, in in vitro collections and/or in liquid nitrogen. Plants, 9(12), 1634.
2 Zuļģe, N., Kāle, A., Gospodaryk, A., Vēvere, K., & Moročko-Bičevska, I. (2017, May). Establishment of nuclear stock collections for apple and pear in Latvia. In Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. (Vol. 71, No. 3, pp. 156-165).
3 Hernández, F., Pinochet, J., Moreno, M. A., Martínez, J. J., & Legua, P. (2010). Performance of Prunus rootstocks for apricot in Mediterranean conditions. Scientia Horticulturae, 124(3), 354-359.
4 Neumüller, M., Mühlberger, L., Siegler, H., Hartmann, W., & Treutter, D. (2012, May). New rootstocks with resistance to Plum pox virus for Prunus domestica and other stone fruit species: the'Docera'and'Dospina'rootstock series. In X International Symposium on Plum and Prune Genetics, Breeding and Pomology 985 (pp. 155-165).
5 Еремин, Г. В., & Еремин, В. Г. (2009). Использование генофонда рода Prunus L. в селекции клоновых подвоев косточковых культур. Биология растений и садоводство: теория, инновации, (131), 90-94.б
6 Hartmann, H. T., Kester, D. E., Davis, F. T., & Geneve, R. L. (2007). Propagation methods and rootstocks for fruit and nut species. In H. T. Hartmann & D. E. Kester (Eds.), Plant propagation: Principal and practice. (pp. 717–757). New York: Pearson Education.
7 Andersen, R., Freer, J., & Robinson, T. (2006). Plum rootstock trial at Geneva: A progress report. New York Fruit Quarterly, 14(1), 27–28.
8 Botu, I., Preda, S., Turcu, E., Achim, G., & Botu, M. (2007). ‘Rival’—A new rootstock for plum. Acta Horticulturae, 732, 253–256.
9 Kumar, A., Rathore, J. P., Iqbal, U., Sharma, A., Nagar, P. K., & Mir, M. M. (2021). Rootstocks of stone fruit crops. Production Technology of Stone Fruits, 131-169.
10 Казыбаева, С. Ж., Уразаева, М. В., & Борисова, А. А. (2021). Современные системы ведения питомниководства республики Казахстан. Плодоводство и ягодоводство России, 66(1), 89-95.
11 Уразаева М., Ефремова Ю., Ормакаев А. Интродуцированные клоновые подвои косточковых / Izdenister natigeler. – 2023. – №2 (98). – С. 143-153. DOI: https://doi.org/10.37884/2-2023/14
12 Certification scheme for almond, apricot, peach and plum. EPPO Bulletin, (2001), 31: 463-478. https://doi.org/10.1111/j.1365-2338.2001.tb01028.x
13 Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures //Physiologia plantarum. – 1962. – Т. 15. – №. 3. – С. 473-497
14 Quoirin M., Lepoivre P. H. Improved media for in vitro culture of Prunus sp //Symposium on Tissue Culture for Horticultural Purposes 78. – 1977. – С. 437-442.
15 Турдиев Т.Т., Ковальчук И. Ю., Успанова Г.К., Чуканова Н.И., Фролов С. Н. Оптимизация клонального микроразмножения для сохранения генофонда растений груши // Вестник КазНУ, Серия биологическая. – 2015. – № 3. – С. 356-362.;
16 Halim, M. A., Alam, M. F., Rahman, M. H., Hossain, M. B., & Uddin, M. B. Sterilization process for In vitro regeneration of Stevia (Stevia rebundiana Bertoni) //International Journal of business, social and scientific research. – 2016. – Т. 4. – №. 4. – Р. 320-323.
17 Беседина Е. Н., Бунцевич Л. Л. Усовершенствования технологии клонального микроразмножения подвоев яблони на этапе введения в культуру in vitro // Политематический сетевой электронный научный журнал Кубанского государственного аграрного университета. – 2015. – №. 111. – с. 1-19.
18 Vujović T. et al. In vitro propagation of plum rootstocks //Voćarstvo: Journal of Pomology. – 2018. – Т. 52. – №. 203-204. – С. 91-97.
19 Супрун И.И., Амосова М.А., Лободина В.Е., Аль-Накиб Е.А., Авакимян А.О., Егорова О.В. Оптимизация этапов клонального микроразмножения подвоев косточковых культур // Научные труды СКФНСЦСВВ. – Т.36. 2023г.

Citation Links

[1]2024. DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS. Izdenister natigeler. 3(103) (Sep. 2024), 86–97. DOI:https://doi.org/10.37884/3-2024/10.

(1)DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS. Izdenister natigeler 2024, No. 3(103), 86-97. https://doi.org/10.37884/3-2024/10.

DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS. (2024). Izdenister Natigeler, 3(103), 86-97. https://doi.org/10.37884/3-2024/10

DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS. Izdenister natigeler, [S. l.], n. 3(103), p. 86–97, 2024. DOI: 10.37884/3-2024/10. Disponível em: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/696. Acesso em: 15 sep. 2026.

“DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS”. 2024. Izdenister Natigeler, no. 3(103) (September): 86-97. https://doi.org/10.37884/3-2024/10.

“DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS” (2024) Izdenister natigeler, (3(103), pp. 86–97. doi:10.37884/3-2024/10.

[1]“DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS”, Izdenister natigeler, no. 3(103), pp. 86–97, Sep. 2024, doi: 10.37884/3-2024/10.

“DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS”. Izdenister Natigeler, no. 3(103), Sept. 2024, pp. 86-97, https://doi.org/10.37884/3-2024/10.

“DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS”. Izdenister natigeler, no. 3(103) (September 30, 2024): 86–97. Accessed September 15, 2026. https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/696.

1.DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS. Izdenister natigeler [Internet]. 2024 Sep. 30 [cited 2026 Sep. 15];(3(103):86-97. Available from: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/696

1.DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS. Izdenister natigeler. 2024;(3(103):86-97. doi:10.37884/3-2024/10

[1] 1 Panis, B., Nagel, M., & Van den Houwe, I. (2020). Challenges and prospects for the conservation of crop genetic resources in field genebanks, in in vitro collections and/or in liquid nitrogen. Plants, 9(12), 1634.

[2] 2 Zuļģe, N., Kāle, A., Gospodaryk, A., Vēvere, K., & Moročko-Bičevska, I. (2017, May). Establishment of nuclear stock collections for apple and pear in Latvia. In Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. (Vol. 71, No. 3, pp. 156-165).

[3] 3 Hernández, F., Pinochet, J., Moreno, M. A., Martínez, J. J., & Legua, P. (2010). Performance of Prunus rootstocks for apricot in Mediterranean conditions. Scientia Horticulturae, 124(3), 354-359.

[4] 4 Neumüller, M., Mühlberger, L., Siegler, H., Hartmann, W., & Treutter, D. (2012, May). New rootstocks with resistance to Plum pox virus for Prunus domestica and other stone fruit species: the'Docera'and'Dospina'rootstock series. In X International Symposium on Plum and Prune Genetics, Breeding and Pomology 985 (pp. 155-165).

[5] 5 Еремин, Г. В., & Еремин, В. Г. (2009). Использование генофонда рода Prunus L. в селекции клоновых подвоев косточковых культур. Биология растений и садоводство: теория, инновации, (131), 90-94.б

[6] 6 Hartmann, H. T., Kester, D. E., Davis, F. T., & Geneve, R. L. (2007). Propagation methods and rootstocks for fruit and nut species. In H. T. Hartmann & D. E. Kester (Eds.), Plant propagation: Principal and practice. (pp. 717–757). New York: Pearson Education.

[7] 7 Andersen, R., Freer, J., & Robinson, T. (2006). Plum rootstock trial at Geneva: A progress report. New York Fruit Quarterly, 14(1), 27–28.

[8] 8 Botu, I., Preda, S., Turcu, E., Achim, G., & Botu, M. (2007). ‘Rival’—A new rootstock for plum. Acta Horticulturae, 732, 253–256.

[9] 9 Kumar, A., Rathore, J. P., Iqbal, U., Sharma, A., Nagar, P. K., & Mir, M. M. (2021). Rootstocks of stone fruit crops. Production Technology of Stone Fruits, 131-169.

[10] 10 Казыбаева, С. Ж., Уразаева, М. В., & Борисова, А. А. (2021). Современные системы ведения питомниководства республики Казахстан. Плодоводство и ягодоводство России, 66(1), 89-95.

[11] 11 Уразаева М., Ефремова Ю., Ормакаев А. Интродуцированные клоновые подвои косточковых / Izdenister natigeler. – 2023. – №2 (98). – С. 143-153. DOI: https://doi.org/10.37884/2-2023/14

[12] 12 Certification scheme for almond, apricot, peach and plum. EPPO Bulletin, (2001), 31: 463-478. https://doi.org/10.1111/j.1365-2338.2001.tb01028.x

[13] 13 Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures //Physiologia plantarum. – 1962. – Т. 15. – №. 3. – С. 473-497

[14] 14 Quoirin M., Lepoivre P. H. Improved media for in vitro culture of Prunus sp //Symposium on Tissue Culture for Horticultural Purposes 78. – 1977. – С. 437-442.

[15] 15 Турдиев Т.Т., Ковальчук И. Ю., Успанова Г.К., Чуканова Н.И., Фролов С. Н. Оптимизация клонального микроразмножения для сохранения генофонда растений груши // Вестник КазНУ, Серия биологическая. – 2015. – № 3. – С. 356-362.;

[16] 16 Halim, M. A., Alam, M. F., Rahman, M. H., Hossain, M. B., & Uddin, M. B. Sterilization process for In vitro regeneration of Stevia (Stevia rebundiana Bertoni) //International Journal of business, social and scientific research. – 2016. – Т. 4. – №. 4. – Р. 320-323.

[17] 17 Беседина Е. Н., Бунцевич Л. Л. Усовершенствования технологии клонального микроразмножения подвоев яблони на этапе введения в культуру in vitro // Политематический сетевой электронный научный журнал Кубанского государственного аграрного университета. – 2015. – №. 111. – с. 1-19.

[18] 18 Vujović T. et al. In vitro propagation of plum rootstocks //Voćarstvo: Journal of Pomology. – 2018. – Т. 52. – №. 203-204. – С. 91-97.

[19] 19 Супрун И.И., Амосова М.А., Лободина В.Е., Аль-Накиб Е.А., Авакимян А.О., Егорова О.В. Оптимизация этапов клонального микроразмножения подвоев косточковых культур // Научные труды СКФНСЦСВВ. – Т.36. 2023г.