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STOCK-RAISING AND VETERINARY

TYPIFICATION OF AVIAN INFLUENZA VIRUS BY RT-PCR

Sh.D. Orkara* 1 , N.Т. Sandybaev 1 , К.К. Tabynov 1

1 Kazakh National Agrarian Research University, Almaty

doi.org/10.37884/4-2021/01 pp. 5-12 Admitted 14.03.2022 Published 30.12.2021

Abstract

Outbreaks of avian influenza among wild and domestic birds are increasingly common worldwide. Over the past 15 years, outbreaks of avian influenza, in particular caused by H5 viruses originating from the A/Goose/Guangdong/1/1996 that originated in Southeast Asia in 1996, have increasingly occurred in Europe and Asia. In the period from 2005 to 2020, at least ten global outbreaks of AIV H5 were detected in the world, which led to the mass death of domestic and wild birds. In recent years, the use of molecular methods for the detection of viral nucleic acids has become an important tool for the detection of avian influenza virus and the identification of hemagglutinin (HA) and neuraminidase (NA) subtypes. These methods include RT-PCR. Reverse transcriptase - polymerase chain reaction is a reaction of DNA copy synthesis (cDNA) on an RNA matrix using a special enzyme - reverse transcriptase, also called revertase. The cDNA is then amplified by polymerase chain reaction (PCR). This article presents the results of typing avian influenza virus by PCR. In 2020, in one of the private farmsteads on the territory of the Almaty region, a case of poultry was noted with symptoms similar to the avian influenza virus. As a result of laboratory studies, the avian influenza virus of the H5 subtype was typed by RT-PCR.

virus, avian influenza, H5, typification, hemagglutinin, influenza subtypes, domestic poultry, RT-PCR

01 Introduction

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02 References

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Citation Links

[1]2021. TYPIFICATION OF AVIAN INFLUENZA VIRUS BY RT-PCR. Izdenister natigeler. 4 (92) (Dec. 2021), 5–12. DOI:https://doi.org/10.37884/4-2021/01.
(1)TYPIFICATION OF AVIAN INFLUENZA VIRUS BY RT-PCR. Izdenister natigeler 2021, No. 4 (92), 5-12. https://doi.org/10.37884/4-2021/01.
TYPIFICATION OF AVIAN INFLUENZA VIRUS BY RT-PCR. (2021). Izdenister Natigeler, 4 (92), 5-12. https://doi.org/10.37884/4-2021/01
TYPIFICATION OF AVIAN INFLUENZA VIRUS BY RT-PCR. Izdenister natigeler, [S. l.], n. 4 (92), p. 5–12, 2021. DOI: 10.37884/4-2021/01. Disponível em: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/60. Acesso em: 15 sep. 2026.
“TYPIFICATION OF AVIAN INFLUENZA VIRUS BY RT-PCR”. 2021. Izdenister Natigeler, no. 4 (92) (December): 5-12. https://doi.org/10.37884/4-2021/01.
“TYPIFICATION OF AVIAN INFLUENZA VIRUS BY RT-PCR” (2021) Izdenister natigeler, (4 (92), pp. 5–12. doi:10.37884/4-2021/01.
[1]“TYPIFICATION OF AVIAN INFLUENZA VIRUS BY RT-PCR”, Izdenister natigeler, no. 4 (92), pp. 5–12, Dec. 2021, doi: 10.37884/4-2021/01.
“TYPIFICATION OF AVIAN INFLUENZA VIRUS BY RT-PCR”. Izdenister Natigeler, no. 4 (92), Dec. 2021, pp. 5-12, https://doi.org/10.37884/4-2021/01.
“TYPIFICATION OF AVIAN INFLUENZA VIRUS BY RT-PCR”. Izdenister natigeler, no. 4 (92) (December 30, 2021): 5–12. Accessed September 15, 2026. https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/60.
1.TYPIFICATION OF AVIAN INFLUENZA VIRUS BY RT-PCR. Izdenister natigeler [Internet]. 2021 Dec. 30 [cited 2026 Sep. 15];(4 (92):5-12. Available from: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/60
1.TYPIFICATION OF AVIAN INFLUENZA VIRUS BY RT-PCR. Izdenister natigeler. 2021;(4 (92):5-12. doi:10.37884/4-2021/01