МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКИЙ АНАЛИЗ УСТОЙЧИВОСТИ ОГУРЦА (CUCUMIS SATIVUS L. К ВОЗБУДИТЕЛЮ ПЕРОНОСПОРОЗА (PSEUDOPERONOSPORA CUBENSIS)

Айгуль Маденова; Еркеназ Кайыпжан; Серик Джантасов; Айгуль Нусупова; Айгерим Джантасова

doi:10.37884/3-2025/32

Abstract

Downy mildew, caused by the pathogen Pseudoperonospora cubensis, is one of the most harmful and widespread diseases of cucumber (Cucumis sativus L.), affecting plants in both open fields and protected environments. The disease has been reported in all major cucumber-growing regions, including Kazakhstan, and can lead to significant reductions in yield and product quality. Under current conditions, the most effective, environmentally safe, and economically justified method of protecting plants from downy mildew is the use of varieties and hybrids with genetic resistance to the pathogen.

The aim of this study was the molecular identification of cucumber samples resistant to downy mildew using PCR analysis and microsatellite (SSR) markers associated with resistance loci. In total, 35 breeding cucumber samples were analyzed. Using SSR markers, an association of resistance with QTL regions Dm5.1 and Dm5.3 was established. The marker CAPs_ENK60, associated with the Dm5.1 locus, identified resistance in 26 samples (74.2%). The second marker, Dm5.3-SSR00772, located in the Dm5.3 region, confirmed resistance in 32 samples (91.4%).

The results demonstrate the high efficiency of using molecular markers in cucumber breeding for resistance to downy mildew. The identified resistant samples are of significant interest for further breeding efforts and can be used in programs to develop new resistant cucumber varieties and hybrids.

cucumber downy mildew resistance SSR markers (Simple Sequence Repeat markers) Dm genes QTL (Quantitative Trait Loci) breeding PCR analysis (Polymerase Chain Reaction analysis)

01 Introduction

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02 References

1. Yang F. et al. Genetic Basis of Agronomic Traits in Cucumber: A Review of QTL Mapping Studies //Molecular Plant Breeding. – 2025. – Т. 16.
2. Шойбекова *, А., Джантасов, С., & Нусипжанов, Н. (2021). Прививка Гибрида Огурца (Лат. Cucumis Sativus) На Подвои Тыквы (Лат. Cucurbita), Устойчивых К Патогену Fusarium. Izdenister Natigeler, (1 (89), 167–176. https://Doi.Org/10.37884/1-2021/18
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5. Kahveci E. et al. Genomic-assisted marker development suitable for Cscvy-1 selection in cucumber breeding //Frontiers in Plant Science. – 2021. – Т. 12. – С. 691576. https://doi.org/10.3389/fpls.2021.691576
6. Bhattarai K. et al. Improvement of crop production in controlled environment agriculture through breeding //Frontiers in Plant Science. – 2025. – Т. 15. – С. 1524601. https://doi.org/10.3389/fpls.2024.1524601
7. Thapliyal V. et al. Utilizing novel parthenocarpic gynoecious cucumber (Cucumis sativus L.) inbreds for exploiting their heterotic potential under poly-house conditions //Euphytica. – 2023. – Т. 219. – №. 9. – С. 99. https://doi.org/10.1007/s10681-023-03229-7
8. Методика государственного сортоиспытания сельскохозяйственных культур. Выпуск первый общая часть. Москва 2019 г.
9. Кулякина Н. В., Юречко Т. К., Кузьмицкая Г. А. Наследник–новый сорт огурца дальневосточной селекции //Овощи России. – 2018. – №. 2. – С. 65-67. https://doi.org/10.18619/2072-9146-2018-2-65-67
10. Лаптев В. Н., Пучков М. Ю. Инновационные сорта и теоретические разработки отдела селекции и биотехнологии овощных культур внииоб и нпп" агровнедрение" за 45 лет //Селекция, семеноводство и технологии выращивания овощных, бахчевых, технических и кормовых культур. – 2014. – №. 1. – С. 5-18. https://doi.org/10.18619/2072-9146-2017-3-3-15
11. Суханбердина Э. Х., Грушин А. А., Пискунова Т. М. Скрининг коллекции огурца по устойчивости к ложной мучнистой росе в зоне Нижнего Поволжья //Труды по прикладной ботанике, генетике и селекции. – 2019. – Т. 180. – №. 2. – С. 102-108. https://doi.org/10.30901/2227-8834-2019-2-102-108
12. Пахратдинова Ж. У., Рсалиев А. С., Амирханова Н. Т. Изучение генетических основ устойчивости сортов огурца к пероноспорозу на основе молекулярно-генетических маркеров //Международный научно-исследовательский журнал. – 2017. – №. 11-3 (65). – С. 85-89. https://Doi.Org/10.37884/1-2021/18
13. Brunborg G., Rolstadaas L., Gutzkow K. B. Electrophoresis in the comet assay //Electrophoresis: Life Sciences Practical Applications. – 2018. – С. 526-652. http://dx.doi.org/10.5772/intechopen.76880

Citation Links

[1]2025. MOLECULAR GENETIC ANALYSIS OF CUCUMBER (CUCUMIS SATIVUS L.) RESISTANCE TO THE DOWNY MILDEW PATHOGEN (PSEUDOPERONOSPORA CUBENSIS). Izdenister natigeler. 3 (107) (Sep. 2025), 335–344. DOI:https://doi.org/10.37884/3-2025/32.

(1)MOLECULAR GENETIC ANALYSIS OF CUCUMBER (CUCUMIS SATIVUS L.) RESISTANCE TO THE DOWNY MILDEW PATHOGEN (PSEUDOPERONOSPORA CUBENSIS). Izdenister natigeler 2025, No. 3 (107), 335-344. https://doi.org/10.37884/3-2025/32.

MOLECULAR GENETIC ANALYSIS OF CUCUMBER (CUCUMIS SATIVUS L.) RESISTANCE TO THE DOWNY MILDEW PATHOGEN (PSEUDOPERONOSPORA CUBENSIS). (2025). Izdenister Natigeler, 3 (107), 335-344. https://doi.org/10.37884/3-2025/32

MOLECULAR GENETIC ANALYSIS OF CUCUMBER (CUCUMIS SATIVUS L.) RESISTANCE TO THE DOWNY MILDEW PATHOGEN (PSEUDOPERONOSPORA CUBENSIS). Izdenister natigeler, [S. l.], n. 3 (107), p. 335–344, 2025. DOI: 10.37884/3-2025/32. Disponível em: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/1061. Acesso em: 15 sep. 2026.

“MOLECULAR GENETIC ANALYSIS OF CUCUMBER (CUCUMIS SATIVUS L.) RESISTANCE TO THE DOWNY MILDEW PATHOGEN (PSEUDOPERONOSPORA CUBENSIS)”. 2025. Izdenister Natigeler, no. 3 (107) (September): 335-44. https://doi.org/10.37884/3-2025/32.

“MOLECULAR GENETIC ANALYSIS OF CUCUMBER (CUCUMIS SATIVUS L.) RESISTANCE TO THE DOWNY MILDEW PATHOGEN (PSEUDOPERONOSPORA CUBENSIS)”. (2025) Izdenister natigeler, (3 (107), pp. 335–344. doi:10.37884/3-2025/32.

[1]“MOLECULAR GENETIC ANALYSIS OF CUCUMBER (CUCUMIS SATIVUS L.) RESISTANCE TO THE DOWNY MILDEW PATHOGEN (PSEUDOPERONOSPORA CUBENSIS)”., Izdenister natigeler, no. 3 (107), pp. 335–344, Sep. 2025, doi: 10.37884/3-2025/32.

“MOLECULAR GENETIC ANALYSIS OF CUCUMBER (CUCUMIS SATIVUS L.) RESISTANCE TO THE DOWNY MILDEW PATHOGEN (PSEUDOPERONOSPORA CUBENSIS)”. Izdenister Natigeler, no. 3 (107), Sept. 2025, pp. 335-44, https://doi.org/10.37884/3-2025/32.

“MOLECULAR GENETIC ANALYSIS OF CUCUMBER (CUCUMIS SATIVUS L.) RESISTANCE TO THE DOWNY MILDEW PATHOGEN (PSEUDOPERONOSPORA CUBENSIS)”. Izdenister natigeler, no. 3 (107) (September 30, 2025): 335–344. Accessed September 15, 2026. https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/1061.

1.MOLECULAR GENETIC ANALYSIS OF CUCUMBER (CUCUMIS SATIVUS L.) RESISTANCE TO THE DOWNY MILDEW PATHOGEN (PSEUDOPERONOSPORA CUBENSIS). Izdenister natigeler [Internet]. 2025 Sep. 30 [cited 2026 Sep. 15];(3 (107):335-44. Available from: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/1061

1.MOLECULAR GENETIC ANALYSIS OF CUCUMBER (CUCUMIS SATIVUS L.) RESISTANCE TO THE DOWNY MILDEW PATHOGEN (PSEUDOPERONOSPORA CUBENSIS). Izdenister natigeler. 2025;(3 (107):335-344. doi:10.37884/3-2025/32

[1] 1. Yang F. et al. Genetic Basis of Agronomic Traits in Cucumber: A Review of QTL Mapping Studies //Molecular Plant Breeding. – 2025. – Т. 16.

[2] 2. Шойбекова *, А., Джантасов, С., & Нусипжанов, Н. (2021). Прививка Гибрида Огурца (Лат. Cucumis Sativus) На Подвои Тыквы (Лат. Cucurbita), Устойчивых К Патогену Fusarium. Izdenister Natigeler, (1 (89), 167–176. https://Doi.Org/10.37884/1-2021/18

[3] 3. Kumari P. et al. Studies on Heterotic Pattern for Earliness, Yield and Quality Traits in Cucumber by Utilizing Monoecious, Gynoecious and Parthenocarpic Lines //Tropical Plant Biology. – 2025. – Т. 18. – №. 1. – С. 23. https://doi.org/10.1007/s12042-024-09390-0

[4] 4. Абзеитова Е.А. и др. Продуктивность тепличного огурца в зависимости от типов почв и норм внесения минеральных удобрений в условиях Юго-Восточного Казахстана. – 2015.

[5] 5. Kahveci E. et al. Genomic-assisted marker development suitable for Cscvy-1 selection in cucumber breeding //Frontiers in Plant Science. – 2021. – Т. 12. – С. 691576. https://doi.org/10.3389/fpls.2021.691576

[6] 6. Bhattarai K. et al. Improvement of crop production in controlled environment agriculture through breeding //Frontiers in Plant Science. – 2025. – Т. 15. – С. 1524601. https://doi.org/10.3389/fpls.2024.1524601

[7] 7. Thapliyal V. et al. Utilizing novel parthenocarpic gynoecious cucumber (Cucumis sativus L.) inbreds for exploiting their heterotic potential under poly-house conditions //Euphytica. – 2023. – Т. 219. – №. 9. – С. 99. https://doi.org/10.1007/s10681-023-03229-7

[8] 8. Методика государственного сортоиспытания сельскохозяйственных культур. Выпуск первый общая часть. Москва 2019 г.

[9] 9. Кулякина Н. В., Юречко Т. К., Кузьмицкая Г. А. Наследник–новый сорт огурца дальневосточной селекции //Овощи России. – 2018. – №. 2. – С. 65-67. https://doi.org/10.18619/2072-9146-2018-2-65-67

[10] 10. Лаптев В. Н., Пучков М. Ю. Инновационные сорта и теоретические разработки отдела селекции и биотехнологии овощных культур внииоб и нпп" агровнедрение" за 45 лет //Селекция, семеноводство и технологии выращивания овощных, бахчевых, технических и кормовых культур. – 2014. – №. 1. – С. 5-18. https://doi.org/10.18619/2072-9146-2017-3-3-15

[11] 11. Суханбердина Э. Х., Грушин А. А., Пискунова Т. М. Скрининг коллекции огурца по устойчивости к ложной мучнистой росе в зоне Нижнего Поволжья //Труды по прикладной ботанике, генетике и селекции. – 2019. – Т. 180. – №. 2. – С. 102-108. https://doi.org/10.30901/2227-8834-2019-2-102-108

[12] 12. Пахратдинова Ж. У., Рсалиев А. С., Амирханова Н. Т. Изучение генетических основ устойчивости сортов огурца к пероноспорозу на основе молекулярно-генетических маркеров //Международный научно-исследовательский журнал. – 2017. – №. 11-3 (65). – С. 85-89. https://Doi.Org/10.37884/1-2021/18

[13] 13. Brunborg G., Rolstadaas L., Gutzkow K. B. Electrophoresis in the comet assay //Electrophoresis: Life Sciences Practical Applications. – 2018. – С. 526-652. http://dx.doi.org/10.5772/intechopen.76880