ТӨМЕН СҰРЫПТЫ КӨМІРДІҢ БЕТІНДЕ ТАРАЛҒАН МИКРОАҒЗАЛАРДЫҢ ФЕРМЕНТАТИВТІ БЕЛСЕНДІЛІГІ

Айнаш Науанова; Эльмира Баимбетова; Гулжанат Максутбекова; Назымгүл Шуменова

doi:10.37884/1-2025/09

Abstract

The article presents the results of a study on the enzymatic activity of microorganisms of the genera Microbacterium, Brevundimonas, Pseudomonas, Streptomyces, and Bacillus, isolated from low-grade brown coal from the Kumiskuduk and Kuznetsk coal mines. These mines are located in the Bukhar-Zhyrau district of the Karaganda region, approximately 35 kilometers east of the city of Karaganda.

To study the enzymatic activity, 10 salt-tolerant strains were selected from the strains isolated on a selective medium with varying concentrations of NaCl. Most of the studied strains isolated from brown coal were capable of synthesizing cellulase, catalase, and pectinase. All salt-tolerant strains, except for strain Microbacterium ginsengiterrae №5, showed high cellulase activity on Hutchinson's medium. All studied strains were capable of producing pectinase, which is important for the decomposition of plant residues in the soil. High catalase activity was observed in strains Microbacterium ginsengiterrae №4, Microbacterium ginsengiterrae №5, Brevundimonas vesicularis №11, Brevundimonas nasdae №12, Brevundimonas vesicularis №19, Pseudomonas mandelii №25, Streptomyces parvus №26, Streptomyces parvus №27, and Brevundimonas vesicularis №28. It was found that strains Streptomyces parvus №27 and Brevundimonas vesicularis №28 had moderate catalase activity, while strain Bacillus firmus №2 did not exhibit this enzyme.

The obtained results highlight that all the listed characteristics of salt-tolerant microorganisms make them promising for the creation of microbiological biological products necessary for increasing the productivity of agricultural crops on saline soils. They also have ecological significance in biotechnological processes aimed at the bioremediation of saline soils and the processing of organic residues and coal waste.

protease activity, catalase activity, cellulase activity, enzymes.

01 Introduction

The full text of the article is available for download in PDF format on the right panel.

02 References

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14. Egamberdieva D., Wirth S., Bellingrath-Kimura S.D., Mishra J., Arora N.K. Salt-Tolerant Plant Growth Promoting Rhizobacteria for Enhancing Crop Productivity of Saline Soils // Front. Microbiol. - 2019. - V.10. 2791.
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19. Terefe M., Tefera T., Sakhuja P.K. Effect of a formulation of Bacillus firmus on root-knot nematode Meloidogyne incognita infestation and the growth of tomato plants in the greenhouse and nursery // Journal of Invertebrate Pathology. - 2009. - V.100. - I. 2. - P. 94-99.

Citation Links

[1]2025. ENZYMATIC ACTIVITY OF DISTRIBUTED MICROORGANISMS ON LOW-GRADE COAL SURFACES. Izdenister natigeler. 1 (105) (Mar. 2025), 71–79. DOI:https://doi.org/10.37884/1-2025/09.

(1)ENZYMATIC ACTIVITY OF DISTRIBUTED MICROORGANISMS ON LOW-GRADE COAL SURFACES. Izdenister natigeler 2025, No. 1 (105), 71-79. https://doi.org/10.37884/1-2025/09.

ENZYMATIC ACTIVITY OF DISTRIBUTED MICROORGANISMS ON LOW-GRADE COAL SURFACES. (2025). Izdenister Natigeler, 1 (105), 71-79. https://doi.org/10.37884/1-2025/09

ENZYMATIC ACTIVITY OF DISTRIBUTED MICROORGANISMS ON LOW-GRADE COAL SURFACES. Izdenister natigeler, [S. l.], n. 1 (105), p. 71–79, 2025. DOI: 10.37884/1-2025/09. Disponível em: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/809. Acesso em: 15 sep. 2026.

“ENZYMATIC ACTIVITY OF DISTRIBUTED MICROORGANISMS ON LOW-GRADE COAL SURFACES”. 2025. Izdenister Natigeler, no. 1 (105) (March): 71-79. https://doi.org/10.37884/1-2025/09.

“ENZYMATIC ACTIVITY OF DISTRIBUTED MICROORGANISMS ON LOW-GRADE COAL SURFACES” (2025) Izdenister natigeler, (1 (105), pp. 71–79. doi:10.37884/1-2025/09.

[1]“ENZYMATIC ACTIVITY OF DISTRIBUTED MICROORGANISMS ON LOW-GRADE COAL SURFACES”, Izdenister natigeler, no. 1 (105), pp. 71–79, Mar. 2025, doi: 10.37884/1-2025/09.

“ENZYMATIC ACTIVITY OF DISTRIBUTED MICROORGANISMS ON LOW-GRADE COAL SURFACES”. Izdenister Natigeler, no. 1 (105), Mar. 2025, pp. 71-79, https://doi.org/10.37884/1-2025/09.

“ENZYMATIC ACTIVITY OF DISTRIBUTED MICROORGANISMS ON LOW-GRADE COAL SURFACES”. Izdenister natigeler, no. 1 (105) (March 30, 2025): 71–79. Accessed September 15, 2026. https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/809.

1.ENZYMATIC ACTIVITY OF DISTRIBUTED MICROORGANISMS ON LOW-GRADE COAL SURFACES. Izdenister natigeler [Internet]. 2025 Mar. 30 [cited 2026 Sep. 15];(1 (105):71-9. Available from: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/809

1.ENZYMATIC ACTIVITY OF DISTRIBUTED MICROORGANISMS ON LOW-GRADE COAL SURFACES. Izdenister natigeler. 2025;(1 (105):71-79. doi:10.37884/1-2025/09

[1] 1.World Bank. Urban Mobility in African Cities: Developing National Urban Mobility Policy and Delivering at the City Level-Summary Report. – 2022.

[2] 2. Furkan H.I., Remzi B.С. Lignocellulosic biomass: a sustainable platform for the production of bio-based chemicals and polymers // Polymer Chemistry. - 2015. - 6. - Р. 4497–4559. doi: 10.1039/C5PY00263J.

[3] 3. Takunda S., Steven J. Medical solid waste management status in Zimbabwe // J. Material of Cycles Waste Managment. - 2023. V.25. - Р. 717-732. 10.1007/s10163-022-01578-4.

[4] 4. Masi C., Gemechu G., Tafesse M. Isolation, screening, characterization, and identification of alkaline protease-producing bacteria from leather industry effluent // Annals of Microbiology. - 2021. – V. 71. https://doi.org/10.1186/s13213-021-01631-x.

[5] 5. Gemechu G., Masi C., Tafesse M., Kebede G. A review on the bacterial alkaline proteases // J Xidian University. - 2020. - V.14(11). - P. 264-274.

[6] 6. Oumer O.J., Abate D. Screening and molecular identification of pectinase producing microbes from coffee pulp // BioMed Research International. - 2018. - V.1. 2961767. https://doi.org/10.1155/2018/2961767, 2-s2.0-85045759257.

[7] 7. Priya S., Sachchida N., Rai S.S., Rachana Ch.AK., Gupta S., Kumar S., Paromita B. Current perspective in research and industrial applications of microbial cellulases // International Journal of Biological Macromolecules. - 2024. - V. 264(1), 130639. https://doi.org/10.1016/j.ijbiomac.2024.130639.

[8] 8. Nitin V., Ankita S., Chetna G., Raja H., Raj Narayan K. Properties of Humic Acid Substances and Their Effect in Soil Quality and Plant Health // Humus and Humic Substances - Recent Advances. Reviewed: - 2022. 10 June 2022.

[9] 9. Свириденко Ю.Я., Свириденко Г.М., Перфильев Г.Д., Захарова М.Б., Матевосян Л.С., Мягконосов Д.С., Шатрова О.А., Оносовская Н.Н., Сёмова Е.Г. Методические рекомендации по организации производственного микробиологического контроля на предприятиях молочной промышленности (с атласом значимых микроорганизмов) МР 2.3.2.2327-08. - 2008.

[10] 10. Sukumaran R.K., Singhania R.R., Pandey А. Microbial cellulases-production, applications and challenges // Journal of Scientific and Industrial Research. - 2005. -V.64(11), 832–844.

[11] 11. Поволоцкая Ю.С. Общее представление о почвенных ферментах // International Journal of Humanities and Natural Sciences. - 2020. - V.1-1 (40). - C. 21-24. DOI: 10.24411/2500-1000-2020-10005.

[12] 12. Jun O., Woro Triarsi S., Qing-Shan L., Hiromi T., Sheng-Xue X., Kenji K., Tokuji I., Sakayu Sh. Two extracellular proteins with alkaline peroxidase activity, a novel cytochrome c and a catalase-peroxidase, from Bacillus sp. No.13 // Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. -2004. - V. 1699, I. 1–2. - P. 65-75.

[13] 13. Akimbekov N.S., Digel I., Tastambek Т.К., Marat А.К., Turaliyeva M.A., Kaiyrmanova G.K. Biotechnology of Microorganisms from Coal Environments: From Environmental Remediation to Energy Production // Biology. - 2022. - V. 11(9), 1306. https://doi.org/10.3390/biology11091306.

[14] 14. Egamberdieva D., Wirth S., Bellingrath-Kimura S.D., Mishra J., Arora N.K. Salt-Tolerant Plant Growth Promoting Rhizobacteria for Enhancing Crop Productivity of Saline Soils // Front. Microbiol. - 2019. - V.10. 2791.

[15] 15. Ekundayo F.O., Oyeniran K.A., Adedokun A.D. Antimicrobial activities of some Streptomyces isolated from garden soil samples and fish pond water in Futa // Journal of Bio-Science. - 2014. - V. 22. DOI:10.3329/jbs.v22i0.30005.

[16] 16.Науанова А.П., Жеделбаева А.С., Алгожина А.Ш., Жакипова А.А. Актиномицеттердің өсуі мен дамуына пестицидтердің әсер ету сипаты //Ізденістер, нәтижелер. – 2024. - №3. – С. 205-213.

[17] 17. Al-Dhabi N.A., Esmail G.A., Mohammed Ghilan A.K., Arasu M.V. Isolation and screening of _Streptomyces_ sp. Al-Dhabi-49 from the environment of Saudi Arabia with concomitant production of lipase and protease in submerged fermentation // Saudi Journal of Biological Sciences. - 2020. - V.27(1). - P. 474–479. https://doi.org/10.1016/j.sjbs.2019.11.011, 31889873.

[18] 18. Moon S.H., Satish J.P. Recombinant protein synthesis and plasmid instability in continuous // Biotechnology and Bioengineering. - 1993. - V.41. I.1. - P. 43-54.

[19] 19. Terefe M., Tefera T., Sakhuja P.K. Effect of a formulation of Bacillus firmus on root-knot nematode Meloidogyne incognita infestation and the growth of tomato plants in the greenhouse and nursery // Journal of Invertebrate Pathology. - 2009. - V.100. - I. 2. - P. 94-99.