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STOCK-RAISING AND VETERINARY

EFFECTIVENESS OF SCHEMES FOR HORMONAL INDUCTION OF POLYOVULATION IN DAIRY CATTLE DONORS IN THE CONDITIONS OF THE SOUTHEASTERN REGION OF KAZAKHSTAN

Dauren Bekenov 1 , Askhat Chindaliyev 2 , Batyrkhan Buralkhiyev 1 , Makpal Kargayeva 3 , Gulzat Gabit 1 , Micinski Jan 4

1 Kazakh National Agrarian Research University; 2 LLP “Research and Production Center for Animal Husbandry and Veterinary Medicine”; 3 Educational Research and Production Center “Baiserke-Agro” LLP; 4 University of Warmia and Mazury in Olsztyn

doi.org/10.37884/2-2024/05 pp. 42-55 Admitted 29.04.2024 Published 29.06.2024

Abstract

The purpose of the work was to study the effectiveness of hormonal induction schemes for polyovulation in donor cattle for dairy production.

One of the most important stages of embryo transplantation technology is hormonal stimulation of superovulation of the ovaries of high-value donor cows, on which the yield of morphologically complete embryos directly depends. Superovulation is a necessary method for obtaining a large number of zygotes for embryo transplantation technology [1]. Traditional methods use follicle-stimulating hormone (FSH) which is administered to donor cattle twice daily for 3-4 days [2]. The superovulation scheme using a complex of hormonal drugs makes it possible to process donors without identifying the heat stage, while the period of hormonal stimulation of donor cows is reduced to 15 days [3].

During the research, the optimal variant of the scheme for hormonal induction of polyovulation in the reproductive cycles of donor cows was selected. Analysis of the results of superovulation showed that the number of ovulations per donor and the yield of full-fledged embryos were higher in animals treated according to schemes No. 1 and No. 2, including the use of the FSH drug and a complex of hormonal drugs - FSH, CIDR, progesterone, estradiol and prostaglandin Treatment of donor cows according to scheme No. 2, providing better fertility of cows after insemination, allows one to obtain a higher (8.3 ± 0.9) yield of full-fledged embryos suitable for transplantation and cryopreservation. The combined use of exogenous hormones in scheme No. 2 allows the treatment of donors without detecting the heat stage, while the time period for hormonal stimulation of donor cows is reduced (up to 15 days).

cattle, dairy farming, embryo transplantation, superovulation, follicle, hormone, embryo.

01 Introduction

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02 References

  1. 1. Hansen PJ. The incompletely fulfilled promise of embryo transfer in cattle-why aren't pregnancy rates greater and what can we do about it? J Anim Sci. 2020 Nov 1;98(11):skaa288. doi: 10.1093/jas/skaa288. PMID: 33141879; PMCID: PMC7608916.
  2. 2. Alireza Khodadadi, Amir Niasari-Naslaji, Darab Nikjou, Behrouz Mohammadi, Superovulation of high-producing Holstein lactating dairy cows with human recombinant FSH and hMG, Theriogenology, Volume 191, 2022, Pages 239-244, ISSN 0093-691X, https://doi.org/10.1016/j.theriogenology.2022.08.010.
  3. 3. Baruselli PS, de Abreu LÂ, Catussi BLC, Oliveira ACDS, Rebeis LM, Gricio EA, Albertini S, Sales JNS, Rodrigues CA. Use of new recombinant proteins for ovarian stimulation in ruminants. Anim Reprod. 2023 Sep 4;20(2):e20230092. doi: 10.1590/1984-3143-AR2023-0092. PMID: 37720727; PMCID: PMC10503889.
  4. 4. Омбаев А., Мирзакулов С., Чиндалиев А.. (2023). НАУЧНО-ТЕХНОЛОГИЧЕСКИЕ АСПЕКТЫ РАЗВИТИЯ ЖИВОТНОВОДСТВА КАЗАХСТАНА. Izdenister Natigeler, (3 (99), 36–48. https://doi.org/10.37884/3-2023/04.
  5. 5. Moore SG, Hasler JF. A 100-Year Review: Reproductive technologies in dairy science. J Dairy Sci. 2017 Dec;100(12):10314-10331. doi: 10.3168/jds.2017-13138. PMID: 29153167.
  6. 6. Currin L, Baldassarre H, Bordignon V. In Vitro Production of Embryos from Prepubertal Holstein Cattle and Mediterranean Water Buffalo: Problems, Progress and Potential. Animals (Basel). 2021 Aug 1;11(8):2275. doi: 10.3390/ani11082275. PMID: 34438733; PMCID: PMC8388507.
  7. 7. Baruselli PS, Ferreira RM, Vieira LM, Souza AH, Bó GA, Rodrigues CA. Use of embryo transfer to alleviate infertility caused by heat stress. Theriogenology. 2020 Oct 1;155:1-11. doi: 10.1016/j.theriogenology.2020.04.028. Epub 2020 Jun 2. PMID: 32562738.
  8. 8. Vasconcelos JL, Jardina DT, Sá Filho OG, Aragon FL, Veras MB. Comparison of progesterone-based protocols with gonadotropin-releasing hormone or estradiol benzoate for timed artificial insemination or embryo transfer in lactating dairy cows. Theriogenology. 2011 Apr 1;75(6):1153-60. doi: 10.1016/j.theriogenology.2010.11.027. Epub 2011 Jan 17. PMID: 21247621.
  9. 9. Hayakawa H, Hirai T, Takimoto A, Ideta A, Aoyagi Y. Superovulation and embryo transfer in Holstein cattle using sexed sperm. Theriogenology. 2009 Jan 1;71(1):68-73. doi: 10.1016/j.theriogenology.2008.09.016. Epub 2008 Oct 31. PMID: 18951623.
  10. 10. Neto AS, Sanches BV, Binelli M, Seneda MM, Perri SH, Garcia JF. Improvement in embryo recovery using double uterine flushing. Theriogenology. 2005 Mar 15;63(5):1249-55. doi: 10.1016/j.theriogenology.2004.03.022. PMID: 15725433.
  11. 11. Rabel RAC, Marchioretto PV, Bangert EA, Wilson K, Milner DJ, Wheeler MB. Pre-Implantation Bovine Embryo Evaluation-From Optics to Omics and Beyond. Animals (Basel). 2023 Jun 24;13(13):2102. doi: 10.3390/ani13132102. PMID: 37443900; PMCID: PMC10339960.
  12. 12. Inaba Y, Miyashita S, Somfai T, Geshi M, Matoba S, Dochi O, Nagai T. Cryopreservation method affects DNA fragmentation in trophectoderm and the speed of re-expansion in bovine blastocysts. Cryobiology. 2016 Apr;72(2):86-92. doi: 10.1016/j.cryobiol.2016.03.006. Epub 2016 Mar 17. PMID: 26996887.
  13. 13. Wang X, Al Naib A, Sun DW, Lonergan P. Membrane permeability characteristics of bovine oocytes and development of a step-wise cryoprotectant adding and diluting protocol. Cryobiology. 2010 Aug;61(1):58-65. doi: 10.1016/j.cryobiol.2010.05.001. Epub 2010 May 12. PMID: 20470768.
  14. 14. Dochi O. Direct transfer of frozen-thawed bovine embryos and its application in cattle reproduction management. J Reprod Dev. 2019 Oct 23;65(5):389-396. doi: 10.1262/jrd.2019-025. Epub 2019 Jun 13. PMID: 31189772; PMCID: PMC6815740.
  15. 15. Selionova MI, Aibazov MM, Zharkova EK. Cryopreservation and Transfer of Sheep Embryos Recovered at Different Stages of Development and Cryopreserved Using Different Techniques. Animals (Basel). 2023 Jul 20;13(14):2361. doi: 10.3390/ani13142361. PMID: 37508138; PMCID: PMC10375972.
  16. 16. Youngs CR. Cryopreservation of preimplantation embryos of cattle, sheep, and goats. J Vis Exp. 2011 Aug 5;(54):2764. doi: 10.3791/2764. PMID: 21847080; PMCID: PMC3211119.
  17. 17. Gómez E, Carrocera S, Martín D, Pérez-Jánez JJ, Prendes J, Prendes JM, Vázquez A, Murillo A, Gimeno I, Muñoz M. Efficient one-step direct transfer to recipients of thawed bovine embryos cultured in vitro and frozen in chemically defined medium. Theriogenology. 2020 Apr 1;146:39-47. doi: 10.1016/j.theriogenology.2020.01.056. Epub 2020 Feb 1. PMID: 32036059.

Citation Links

[1]2024. EFFECTIVENESS OF SCHEMES FOR HORMONAL INDUCTION OF POLYOVULATION IN DAIRY CATTLE DONORS IN THE CONDITIONS OF THE SOUTHEASTERN REGION OF KAZAKHSTAN. Izdenister natigeler. 2 (102) (Jun. 2024), 42–55. DOI:https://doi.org/10.37884/2-2024/05.
(1)EFFECTIVENESS OF SCHEMES FOR HORMONAL INDUCTION OF POLYOVULATION IN DAIRY CATTLE DONORS IN THE CONDITIONS OF THE SOUTHEASTERN REGION OF KAZAKHSTAN. Izdenister natigeler 2024, No. 2 (102), 42-55. https://doi.org/10.37884/2-2024/05.
EFFECTIVENESS OF SCHEMES FOR HORMONAL INDUCTION OF POLYOVULATION IN DAIRY CATTLE DONORS IN THE CONDITIONS OF THE SOUTHEASTERN REGION OF KAZAKHSTAN. (2024). Izdenister Natigeler, 2 (102), 42-55. https://doi.org/10.37884/2-2024/05
EFFECTIVENESS OF SCHEMES FOR HORMONAL INDUCTION OF POLYOVULATION IN DAIRY CATTLE DONORS IN THE CONDITIONS OF THE SOUTHEASTERN REGION OF KAZAKHSTAN. Izdenister natigeler, [S. l.], n. 2 (102), p. 42–55, 2024. DOI: 10.37884/2-2024/05. Disponível em: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/608. Acesso em: 15 sep. 2026.
“EFFECTIVENESS OF SCHEMES FOR HORMONAL INDUCTION OF POLYOVULATION IN DAIRY CATTLE DONORS IN THE CONDITIONS OF THE SOUTHEASTERN REGION OF KAZAKHSTAN”. 2024. Izdenister Natigeler, no. 2 (102) (June): 42-55. https://doi.org/10.37884/2-2024/05.
“EFFECTIVENESS OF SCHEMES FOR HORMONAL INDUCTION OF POLYOVULATION IN DAIRY CATTLE DONORS IN THE CONDITIONS OF THE SOUTHEASTERN REGION OF KAZAKHSTAN” (2024) Izdenister natigeler, (2 (102), pp. 42–55. doi:10.37884/2-2024/05.
[1]“EFFECTIVENESS OF SCHEMES FOR HORMONAL INDUCTION OF POLYOVULATION IN DAIRY CATTLE DONORS IN THE CONDITIONS OF THE SOUTHEASTERN REGION OF KAZAKHSTAN”, Izdenister natigeler, no. 2 (102), pp. 42–55, Jun. 2024, doi: 10.37884/2-2024/05.
“EFFECTIVENESS OF SCHEMES FOR HORMONAL INDUCTION OF POLYOVULATION IN DAIRY CATTLE DONORS IN THE CONDITIONS OF THE SOUTHEASTERN REGION OF KAZAKHSTAN”. Izdenister Natigeler, no. 2 (102), June 2024, pp. 42-55, https://doi.org/10.37884/2-2024/05.
“EFFECTIVENESS OF SCHEMES FOR HORMONAL INDUCTION OF POLYOVULATION IN DAIRY CATTLE DONORS IN THE CONDITIONS OF THE SOUTHEASTERN REGION OF KAZAKHSTAN”. Izdenister natigeler, no. 2 (102) (June 29, 2024): 42–55. Accessed September 15, 2026. https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/608.
1.EFFECTIVENESS OF SCHEMES FOR HORMONAL INDUCTION OF POLYOVULATION IN DAIRY CATTLE DONORS IN THE CONDITIONS OF THE SOUTHEASTERN REGION OF KAZAKHSTAN. Izdenister natigeler [Internet]. 2024 Jun. 29 [cited 2026 Sep. 15];(2 (102):42-55. Available from: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/608
1.EFFECTIVENESS OF SCHEMES FOR HORMONAL INDUCTION OF POLYOVULATION IN DAIRY CATTLE DONORS IN THE CONDITIONS OF THE SOUTHEASTERN REGION OF KAZAKHSTAN. Izdenister natigeler. 2024;(2 (102):42-55. doi:10.37884/2-2024/05