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AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

PROTEIN AND MOLECULAR MARKERS IN THE QUALITY PREDICTION OF WESTERN PAMIR WHEAT LANDRACE VARIETIES

Dilyara Babissekova 1 , Shynar Mazkirat 2 , Sholpan Khalbaeva 1 , Kozimamad Abdulamonov 3 , Akzhan Espembetova 1 , Azamat Tukenov 1 , Kulpash Bulatova 1

1 Kazakh Research Institute of Agriculture and Plant Growing LLP; 2 LLP "Kazakh Research Institute of Agriculture and Plant Growing"; 3 Pamir Biological Institute named after. H.Yu. Yusufbekova

doi.org/10.37884/2-1-2024/537 pp. 34-45 Admitted 17.04.2024 Published 29.06.2024

Abstract

Preservation of the genetic diversity of cultivated varieties and hybrids of agricultural crops is one of the most important tasks of agricultural science, contributing to the stable production of crop products. One possible source of valuable traits is landraces still cultivated in different eco-geographical conditions. They represent an untouched reserve of diversity, sources of resistance to unfavorable growing conditions, and properties that improve the quality of plant products.

The article presents the results of studies the Western Pamirs (Tajikistan, Afghanistan) 9 landraces on protein and molecular markers associated with the quality of grain and wheat flour.

The method of the seeds storage protein - gliadin electrophoresis revealed the specificity of all varieties, with the exception of Sadirak belokolosy and Sadirak krasnokolosy, which were identical on protein spectrum.

Analysis of the composition of high-molecular-weight glutenin subunits (HMWGS) showed that all analyzed varieties had a null-c allele of the Glu-A1 locus, with the exception of the varieties Safedax Ishkashimsky and Bludon. All landraces carried the b allele of the Glu-B1 locus, responsible for the biosynthesis of HMWGS 7+8. According to the composition of HMWGSs controlled by the Glu-D1 locus, all samples had the subunit pair 2+12 (allele a of this locus).

Molecular (DNA) markers confirmed the HMWGS composition of the studied varieties, which were identified by protein electrophoresis.

The varieties Safedak Ishkashimsky and Bludon have the highest quality rating for glutenin composition.

PCR analysis revealed alleles Pina-D1a and Pinb-D1a (wild type), characteristic of soft grain wheat, in all landraces.

Genotyping of landraces according to the allelic composition of waxy genes did not reveal carriers of mutant, null alleles, all landraces were characterized by a typical ratio of two starch fractions.

landrace, wheat, Pamir, quality, protein, molecular markers, grain hardness, waxy genes.

01 Introduction

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02 References

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Citation Links

[1]2024. PROTEIN AND MOLECULAR MARKERS IN THE QUALITY PREDICTION OF WESTERN PAMIR WHEAT LANDRACE VARIETIES. Izdenister natigeler. 2-1 (special) (Jun. 2024), 34–45. DOI:https://doi.org/10.37884/2-1-2024/537.
(1)PROTEIN AND MOLECULAR MARKERS IN THE QUALITY PREDICTION OF WESTERN PAMIR WHEAT LANDRACE VARIETIES. Izdenister natigeler 2024, No. 2-1 (special), 34-45. https://doi.org/10.37884/2-1-2024/537.
PROTEIN AND MOLECULAR MARKERS IN THE QUALITY PREDICTION OF WESTERN PAMIR WHEAT LANDRACE VARIETIES. (2024). Izdenister Natigeler, 2-1 (special), 34-45. https://doi.org/10.37884/2-1-2024/537
PROTEIN AND MOLECULAR MARKERS IN THE QUALITY PREDICTION OF WESTERN PAMIR WHEAT LANDRACE VARIETIES. Izdenister natigeler, [S. l.], n. 2-1 (special), p. 34–45, 2024. DOI: 10.37884/2-1-2024/537. Disponível em: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/537. Acesso em: 15 sep. 2026.
“PROTEIN AND MOLECULAR MARKERS IN THE QUALITY PREDICTION OF WESTERN PAMIR WHEAT LANDRACE VARIETIES”. 2024. Izdenister Natigeler, no. 2-1 (special) (June): 34-45. https://doi.org/10.37884/2-1-2024/537.
“PROTEIN AND MOLECULAR MARKERS IN THE QUALITY PREDICTION OF WESTERN PAMIR WHEAT LANDRACE VARIETIES” (2024) Izdenister natigeler, (2-1 (special), pp. 34–45. doi:10.37884/2-1-2024/537.
[1]“PROTEIN AND MOLECULAR MARKERS IN THE QUALITY PREDICTION OF WESTERN PAMIR WHEAT LANDRACE VARIETIES”, Izdenister natigeler, no. 2-1, pp. 34–45, Jun. 2024, doi: 10.37884/2-1-2024/537.
“PROTEIN AND MOLECULAR MARKERS IN THE QUALITY PREDICTION OF WESTERN PAMIR WHEAT LANDRACE VARIETIES”. Izdenister Natigeler, no. 2-1 (special), June 2024, pp. 34-45, https://doi.org/10.37884/2-1-2024/537.
“PROTEIN AND MOLECULAR MARKERS IN THE QUALITY PREDICTION OF WESTERN PAMIR WHEAT LANDRACE VARIETIES”. Izdenister natigeler, no. 2-1 (special) (June 29, 2024): 34–45. Accessed September 15, 2026. https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/537.
1.PROTEIN AND MOLECULAR MARKERS IN THE QUALITY PREDICTION OF WESTERN PAMIR WHEAT LANDRACE VARIETIES. Izdenister natigeler [Internet]. 2024 Jun. 29 [cited 2026 Sep. 15];(2-1 (special):34-45. Available from: https://agrosoil.kaznaru.edu.kz/index.php/research/article/view/537
1.PROTEIN AND MOLECULAR MARKERS IN THE QUALITY PREDICTION OF WESTERN PAMIR WHEAT LANDRACE VARIETIES. Izdenister natigeler. 2024;(2-1 (special):34-45. doi:10.37884/2-1-2024/537